Oxidized LDL induce hsp70 expression in human smooth muscle cells

W. M. Zhu, P. Roma, A. Pirillo, F. Pellegatta, A. L. Catapano

Research output: Contribution to journalArticlepeer-review

Abstract

Heat shock protein 70 (hsp70) has been detected in atherosclerotic lesions, in which endothelial cells and smooth muscle cells are involved. In a previous report we showed that Ox-LDL, a causal factor in atherosclerosis, could induce hsp70 expression in cultured human endothelial cells [Zhu et al. B.B.R.C 1994, 200: 389]. Here, with immunofluorescence and immunoblotting techniques, we show that Ox-LDL are capable of inducing hsp70 expression also in human smooth muscle cells, and that this induction is dependent on cell density and on the concentration of Ox-LDL. The induced expression of hsp70 was higher in human umbilical vein smooth muscle cells than in a human smooth muscle cell line. Conversely, Ox-LDL was cytotoxic to both types of cells, more so to the human smooth muscle cell line. These observations indicate that Ox-LDL may be a stress responsible for hsp70 expression in atherosclerotic plaques and the presence of hsp70 in plaques may be a useful marker for continuous oxidative damage in the arterial wall.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalFEBS Letters
Volume372
Issue number1
DOIs
Publication statusPublished - Sep 18 1995

Keywords

  • Atherosclerosis
  • Cytotoxicity
  • Heat shock protein 70
  • Human smooth cell
  • Oxidized-LDL

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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