Abstract
Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation 'in vitro' with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endothelial cells (HUVEC). This induction was parallel to the cytotoxicity of oxidized LDL as determined by [3H] adenine release. When cells were confluent, however, both effects were greatly reduced. We speculate that induction of hsp70 is related to the citotoxicity of oxidized LDL and that the detection of heat shock proteins in human atherosclerotic plaques is a further indication for the presence 'in vivo' of oxidized LDL. These observations may be relevant to the understanding of endothelial response to injury in proatherosclerotic events.
| Original language | English |
|---|---|
| Pages (from-to) | 389-394 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 200 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1994 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells. / Zhu, W.; Roma, P.; Pellegatta, F.; Catapano, A. L.
In: Biochemical and Biophysical Research Communications, Vol. 200, No. 1, 1994, p. 389-394.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells
AU - Zhu, W.
AU - Roma, P.
AU - Pellegatta, F.
AU - Catapano, A. L.
PY - 1994
Y1 - 1994
N2 - Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation 'in vitro' with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endothelial cells (HUVEC). This induction was parallel to the cytotoxicity of oxidized LDL as determined by [3H] adenine release. When cells were confluent, however, both effects were greatly reduced. We speculate that induction of hsp70 is related to the citotoxicity of oxidized LDL and that the detection of heat shock proteins in human atherosclerotic plaques is a further indication for the presence 'in vivo' of oxidized LDL. These observations may be relevant to the understanding of endothelial response to injury in proatherosclerotic events.
AB - Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation 'in vitro' with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endothelial cells (HUVEC). This induction was parallel to the cytotoxicity of oxidized LDL as determined by [3H] adenine release. When cells were confluent, however, both effects were greatly reduced. We speculate that induction of hsp70 is related to the citotoxicity of oxidized LDL and that the detection of heat shock proteins in human atherosclerotic plaques is a further indication for the presence 'in vivo' of oxidized LDL. These observations may be relevant to the understanding of endothelial response to injury in proatherosclerotic events.
UR - http://www.scopus.com/inward/record.url?scp=0028233741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028233741&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.1461
DO - 10.1006/bbrc.1994.1461
M3 - Article
C2 - 8166710
AN - SCOPUS:0028233741
VL - 200
SP - 389
EP - 394
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -