Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells

W. Zhu, P. Roma, F. Pellegatta, A. L. Catapano

Research output: Contribution to journalArticle

Abstract

Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation 'in vitro' with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endothelial cells (HUVEC). This induction was parallel to the cytotoxicity of oxidized LDL as determined by [3H] adenine release. When cells were confluent, however, both effects were greatly reduced. We speculate that induction of hsp70 is related to the citotoxicity of oxidized LDL and that the detection of heat shock proteins in human atherosclerotic plaques is a further indication for the presence 'in vivo' of oxidized LDL. These observations may be relevant to the understanding of endothelial response to injury in proatherosclerotic events.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume200
Issue number1
DOIs
Publication statusPublished - 1994

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HSP70 Heat-Shock Proteins
Endothelial cells
Endothelial Cells
Atherosclerotic Plaques
Heat-Shock Proteins
Human Umbilical Vein Endothelial Cells
Adenine
Cytotoxicity
Fluorescent Antibody Technique
Cells
Cell Line
oxidized low density lipoprotein
Wounds and Injuries

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells. / Zhu, W.; Roma, P.; Pellegatta, F.; Catapano, A. L.

In: Biochemical and Biophysical Research Communications, Vol. 200, No. 1, 1994, p. 389-394.

Research output: Contribution to journalArticle

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