P-selectin is expressed on the oolemma of human and hamster oocytes following sperm adhesion and is also detected on the equatorial region of acrosome-reacted human spermatozoa

F. M. Fusi, M. Montesano, N. Bernocchi, C. Panzeri, F. Ferrara, A. Villa, R. A. Bronson

Research output: Contribution to journalArticlepeer-review

Abstract

Selectins are a family of adhesive molecules, involved in the interactions between leukocytes and endothelium and in platelet adhesion. P-selectin, one of the members of this family, is stored in α-granules and dense granules of platelets as well as in Weibel-Palade bodies of endothelial cells, and it is rapidly redistributed to the cell surface after activation. It recognizes carbohydrate structures as ligands, in particular sialyl-Lewisx, which is part of the CD15 antigen. In this work we studied P-selectin expression on gametes. While zonafree human and hamster oocytes did not react with a monoclonal antibody directed against P-selectin, oocytes from both species displayed a reactivity with this antibody following their contact with human spermatozoa, as demonstrated both by covasphere binding and indirect immunofluorescence. Artificial activation of zona-intact human oocytes by means of the calcium ionophore A23187 induced the expression on the oolemma of a moiety reacting with anti-P-selectin antibody as well. P-selectin also appeared to be expressed on the sperm surface following the acrosome reaction, as demonstrated by a flow cytometric study of reactivity of spermatozoa with the anti-P-selectin antibody, using the expression of CD46 as a marker of the acrosome reaction. The localization of the P-selectin moiety on the equatorial region of the plasma membrane of acrosome reacted spermatozoa was confirmed by transmission electron microscopy using immunogold labelling. We suggest that P-selectin might be involved in gamete interactions.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalMolecular Human Reproduction
Volume2
Issue number5
Publication statusPublished - May 1996

Keywords

  • Acrosome reaction
  • Gamete interaction
  • Oolemma
  • P-selectin
  • Spermatozoa

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Genetics
  • Embryology
  • Obstetrics and Gynaecology

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