Abstract
Selectins are a family of adhesive molecules, involved in the interactions between leukocytes and endothelium and in platelet adhesion. P-selectin, one of the members of this family, is stored in α-granules and dense granules of platelets as well as in Weibel-Palade bodies of endothelial cells, and it is rapidly redistributed to the cell surface after activation. It recognizes carbohydrate structures as ligands, in particular sialyl-Lewisx, which is part of the CD15 antigen. In this work we studied P-selectin expression on gametes. While zonafree human and hamster oocytes did not react with a monoclonal antibody directed against P-selectin, oocytes from both species displayed a reactivity with this antibody following their contact with human spermatozoa, as demonstrated both by covasphere binding and indirect immunofluorescence. Artificial activation of zona-intact human oocytes by means of the calcium ionophore A23187 induced the expression on the oolemma of a moiety reacting with anti-P-selectin antibody as well. P-selectin also appeared to be expressed on the sperm surface following the acrosome reaction, as demonstrated by a flow cytometric study of reactivity of spermatozoa with the anti-P-selectin antibody, using the expression of CD46 as a marker of the acrosome reaction. The localization of the P-selectin moiety on the equatorial region of the plasma membrane of acrosome reacted spermatozoa was confirmed by transmission electron microscopy using immunogold labelling. We suggest that P-selectin might be involved in gamete interactions.
Original language | English |
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Pages (from-to) | 341-347 |
Number of pages | 7 |
Journal | Molecular Human Reproduction |
Volume | 2 |
Issue number | 5 |
Publication status | Published - May 1996 |
Keywords
- Acrosome reaction
- Gamete interaction
- Oolemma
- P-selectin
- Spermatozoa
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Genetics
- Embryology
- Obstetrics and Gynaecology