p14ARF interacts with N-Myc and inhibits its transcriptional activity

Stefano Amente; Barbara Gargano; Daniel Diolaiti; Giuliano Della Valle; Luigi Lania; Barbara Majello

doi:10.1016/j.febslet.2007.01.062

p14ARF interacts with N-Myc and inhibits its transcriptional activity

Stefano Amente, Barbara Gargano, Daniel Diolaiti, Giuliano Della Valle, Luigi Lania, Barbara Majello

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we report that the human p14^ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14^ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14^ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14^ARF protein is involved in binding to c-Myc and N-Myc proteins.

Original language	English
Pages (from-to)	821-825
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2007.01.062
Publication status	Published - Mar 6 2007

Keywords

C-Myc
N-Myc
p14
Protein interactions
Transcription
Tumor suppressor

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2007.01.062

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title = "p14ARF interacts with N-Myc and inhibits its transcriptional activity",

abstract = "In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.",

keywords = "C-Myc, N-Myc, p14, Protein interactions, Transcription, Tumor suppressor",

author = "Stefano Amente and Barbara Gargano and Daniel Diolaiti and {Della Valle}, Giuliano and Luigi Lania and Barbara Majello",

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T1 - p14ARF interacts with N-Myc and inhibits its transcriptional activity

AU - Amente, Stefano

AU - Gargano, Barbara

AU - Diolaiti, Daniel

AU - Della Valle, Giuliano

AU - Lania, Luigi

AU - Majello, Barbara

PY - 2007/3/6

Y1 - 2007/3/6

N2 - In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.

AB - In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.

KW - C-Myc

KW - N-Myc

KW - p14

KW - Protein interactions

KW - Transcription

KW - Tumor suppressor

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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ER -

p14ARF interacts with N-Myc and inhibits its transcriptional activity

Abstract

Keywords

ASJC Scopus subject areas

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