Abstract
In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.
Original language | English |
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Pages (from-to) | 821-825 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 581 |
Issue number | 5 |
DOIs | |
Publication status | Published - Mar 6 2007 |
Keywords
- C-Myc
- N-Myc
- p14
- Protein interactions
- Transcription
- Tumor suppressor
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology