p14ARF interacts with N-Myc and inhibits its transcriptional activity

Stefano Amente, Barbara Gargano, Daniel Diolaiti, Giuliano Della Valle, Luigi Lania, Barbara Majello

Research output: Contribution to journalArticle

Abstract

In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.

Original languageEnglish
Pages (from-to)821-825
Number of pages5
JournalFEBS Letters
Volume581
Issue number5
DOIs
Publication statusPublished - Mar 6 2007

Keywords

  • C-Myc
  • N-Myc
  • p14
  • Protein interactions
  • Transcription
  • Tumor suppressor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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