p14ARF interacts with N-Myc and inhibits its transcriptional activity

Stefano Amente, Barbara Gargano, Daniel Diolaiti, Giuliano Della Valle, Luigi Lania, Barbara Majello

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140-300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.

Original languageEnglish
Pages (from-to)821-825
Number of pages5
JournalFEBS Letters
Volume581
Issue number5
DOIs
Publication statusPublished - Mar 6 2007

Fingerprint

Tumor Suppressor Protein p14ARF
Proto-Oncogene Proteins c-myc
Neuroblastoma
Transcriptional Activation
Chemical activation
Cells
Cell Line

Keywords

  • C-Myc
  • N-Myc
  • p14
  • Protein interactions
  • Transcription
  • Tumor suppressor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Amente, S., Gargano, B., Diolaiti, D., Della Valle, G., Lania, L., & Majello, B. (2007). p14ARF interacts with N-Myc and inhibits its transcriptional activity. FEBS Letters, 581(5), 821-825. https://doi.org/10.1016/j.febslet.2007.01.062

p14ARF interacts with N-Myc and inhibits its transcriptional activity. / Amente, Stefano; Gargano, Barbara; Diolaiti, Daniel; Della Valle, Giuliano; Lania, Luigi; Majello, Barbara.

In: FEBS Letters, Vol. 581, No. 5, 06.03.2007, p. 821-825.

Research output: Contribution to journalArticle

Amente, S, Gargano, B, Diolaiti, D, Della Valle, G, Lania, L & Majello, B 2007, 'p14ARF interacts with N-Myc and inhibits its transcriptional activity', FEBS Letters, vol. 581, no. 5, pp. 821-825. https://doi.org/10.1016/j.febslet.2007.01.062
Amente S, Gargano B, Diolaiti D, Della Valle G, Lania L, Majello B. p14ARF interacts with N-Myc and inhibits its transcriptional activity. FEBS Letters. 2007 Mar 6;581(5):821-825. https://doi.org/10.1016/j.febslet.2007.01.062
Amente, Stefano ; Gargano, Barbara ; Diolaiti, Daniel ; Della Valle, Giuliano ; Lania, Luigi ; Majello, Barbara. / p14ARF interacts with N-Myc and inhibits its transcriptional activity. In: FEBS Letters. 2007 ; Vol. 581, No. 5. pp. 821-825.
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