p14ARF is capable of promoting HIV-1 tat degradation

Barbara Gargano, Marianna Fiorillo, Stefano Amente, Barbara Majello, Luigi Lania

Research output: Contribution to journalArticlepeer-review

Abstract

The p14ARF tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14 ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14 ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.

Original languageEnglish
Pages (from-to)1433-1439
Number of pages7
JournalCell Cycle
Volume7
Issue number10
Publication statusPublished - May 15 2008

Keywords

  • Degradation
  • Gene expression
  • Inhibition
  • Oncosuppressor
  • p14
  • Tat
  • Ubiquitination

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

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