p14ARF is capable of promoting HIV-1 tat degradation

Barbara Gargano; Marianna Fiorillo; Stefano Amente; Barbara Majello; Luigi Lania

p14ARF is capable of promoting HIV-1 tat degradation

Barbara Gargano, Marianna Fiorillo, Stefano Amente, Barbara Majello, Luigi Lania

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

The p14^ARF tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14 ^ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14 ^ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.

Original language	English
Pages (from-to)	1433-1439
Number of pages	7
Journal	Cell Cycle
Volume	7
Issue number	10
Publication status	Published - May 15 2008

Keywords

Degradation
Gene expression
Inhibition
Oncosuppressor
p14
Tat
Ubiquitination

ASJC Scopus subject areas

Cell Biology
Biochemistry
Molecular Biology
Medicine(all)

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abstract = "The p14ARF tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14 ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14 ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.",

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T1 - p14ARF is capable of promoting HIV-1 tat degradation

AU - Gargano, Barbara

AU - Fiorillo, Marianna

AU - Amente, Stefano

AU - Majello, Barbara

AU - Lania, Luigi

PY - 2008/5/15

Y1 - 2008/5/15

N2 - The p14ARF tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14 ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14 ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.

AB - The p14ARF tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14 ARF inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14 ARF inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.

KW - Degradation

KW - Gene expression

KW - Inhibition

KW - Oncosuppressor

KW - p14

KW - Tat

KW - Ubiquitination

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p14ARF is capable of promoting HIV-1 tat degradation

Abstract

Keywords

ASJC Scopus subject areas

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