P21 RNA and protein expression in non-small cell lung carcinomas

Evidence of p53-independent expression and association with tumoral differentiation

Antonio Marchetti, Claudio Doglioni, Mattia Barbareschi, Fiamma Buttitta, Silvia Pellegrini, Gloria Bertacca, Antonio Chella, Giorgio Merlo, Carlo Alberto Angeletti, Paolo Dalla Palma, Generoso Bevilacqua

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

p21, the product of the WAF1/CIP1/SDI1/mda-6 gene, is an inhibitor of cyclin-dependent kinases. In cell cultures p21 is induced by p53-dependent and p53-independent pathways by DNA damage and induction of differentiation. We investigated p21 RNA and immuno-histochemical expression in 43 non-small cell lung carcinomas and corresponding normal lung samples previously investigated for p53 and WAF1 gene status and p53 protein expression. p21 RNA and protein expression in normal and neoplastic tissues were strictly associated (P = 0.0001). In normal tissue p21 RNA was expressed at low levels and p21 immunoreactivity was seen in scattered differentiated bronchial, alveolar and stromal cells. In the majority of neoplasms p21 protein and RNA were expressed at higher levels than in the corresponding normal tissues: p21 overexpression was seen in 27 (63%) and 28 (65%) cases respectively. p21 was expressed independently from p53 gene/protein alterations. p21 overexpression was more frequent in well differentiated tumors (P = 0.01 and P = 0.022 for RNA and protein respectively), and p21 immunoreactivity was usually seen in foci of more pronounced differentiation. We conclude that p21 expression is related to tumor differentiation, and that p53-independent p21 expression is a common feature of in vivo neoplasms.

Original languageEnglish
Pages (from-to)1319-1324
Number of pages6
JournalOncogene
Volume12
Issue number6
Publication statusPublished - 1996

Fingerprint

Non-Small Cell Lung Carcinoma
RNA
Neoplasm RNA
p53 Genes
Neoplasm Proteins
Proteins
Alveolar Epithelial Cells
Neoplasms
Cyclin-Dependent Kinases
Stromal Cells
DNA Damage
Cell Culture Techniques
Lung
Genes

Keywords

  • CIP1
  • Differentiation
  • Histology
  • Lung cancer
  • mda-6
  • p21
  • p53
  • SDI1
  • WAF1

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Marchetti, A., Doglioni, C., Barbareschi, M., Buttitta, F., Pellegrini, S., Bertacca, G., ... Bevilacqua, G. (1996). P21 RNA and protein expression in non-small cell lung carcinomas: Evidence of p53-independent expression and association with tumoral differentiation. Oncogene, 12(6), 1319-1324.

P21 RNA and protein expression in non-small cell lung carcinomas : Evidence of p53-independent expression and association with tumoral differentiation. / Marchetti, Antonio; Doglioni, Claudio; Barbareschi, Mattia; Buttitta, Fiamma; Pellegrini, Silvia; Bertacca, Gloria; Chella, Antonio; Merlo, Giorgio; Angeletti, Carlo Alberto; Palma, Paolo Dalla; Bevilacqua, Generoso.

In: Oncogene, Vol. 12, No. 6, 1996, p. 1319-1324.

Research output: Contribution to journalArticle

Marchetti, A, Doglioni, C, Barbareschi, M, Buttitta, F, Pellegrini, S, Bertacca, G, Chella, A, Merlo, G, Angeletti, CA, Palma, PD & Bevilacqua, G 1996, 'P21 RNA and protein expression in non-small cell lung carcinomas: Evidence of p53-independent expression and association with tumoral differentiation', Oncogene, vol. 12, no. 6, pp. 1319-1324.
Marchetti, Antonio ; Doglioni, Claudio ; Barbareschi, Mattia ; Buttitta, Fiamma ; Pellegrini, Silvia ; Bertacca, Gloria ; Chella, Antonio ; Merlo, Giorgio ; Angeletti, Carlo Alberto ; Palma, Paolo Dalla ; Bevilacqua, Generoso. / P21 RNA and protein expression in non-small cell lung carcinomas : Evidence of p53-independent expression and association with tumoral differentiation. In: Oncogene. 1996 ; Vol. 12, No. 6. pp. 1319-1324.
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AU - Bertacca, Gloria

AU - Chella, Antonio

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