p21/WAF1/CIP1 expression in normal mucosa and in adenomas and adenocarcinomas of the colon

Its relationship with differentiation

Claudio Doglioni, Paola Pelosio, Licia Laurino, Ettore Macri, Enzo Meggiolaro, Franco Favretti, Mattia Barbareschi

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

The immunoreactivity of p21 was evaluated in normal mucosa and in adenomas and adenocarcinomas of the human large bowel. In normal mucosa, p21 immunoreactivity was seen in the superficial third of the crypts (maturation compartment) and in surface (terminally differentiated) epithelium, and was mutually exclusive with Ki67/MIB1 reactivity. These data show that p21 expression is inversely related to proliferation and directly related to terminal differentiation. In adenomas, p21-reactive cells were frequently clustered in the superficial areas and were non-reactive for MIB1. In adenocarcinomas, p21 staining was heterogeneous: high p21 expression (19 cases) was associated with lower stage and lack of p53 overexpression. p21-reactive cells were devoid of MIB1 immunoreactivity, but no relationship could be found between p21 and MIB1 labelling indices. p21 heterogeneity may be related to alterations in the p53-dependent induction pathway: high p21 expression was associated with low to absent p53 reactivity, with presumed normal p53 function; low p21 expression was associated with p53 overexpression, with presumed p53 alteration resulting in loss of function.

Original languageEnglish
Pages (from-to)248-253
Number of pages6
JournalJournal of Pathology
Volume179
Issue number3
DOIs
Publication statusPublished - 1996

Fingerprint

Adenoma
Colon
Mucous Membrane
Adenocarcinoma
Epithelium
Staining and Labeling

Keywords

  • colon
  • cyclin-dependent inhibitor
  • differentiation
  • human
  • immunohistochemistry
  • neoplasm
  • p21
  • p53
  • proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

p21/WAF1/CIP1 expression in normal mucosa and in adenomas and adenocarcinomas of the colon : Its relationship with differentiation. / Doglioni, Claudio; Pelosio, Paola; Laurino, Licia; Macri, Ettore; Meggiolaro, Enzo; Favretti, Franco; Barbareschi, Mattia.

In: Journal of Pathology, Vol. 179, No. 3, 1996, p. 248-253.

Research output: Contribution to journalArticle

Doglioni, Claudio ; Pelosio, Paola ; Laurino, Licia ; Macri, Ettore ; Meggiolaro, Enzo ; Favretti, Franco ; Barbareschi, Mattia. / p21/WAF1/CIP1 expression in normal mucosa and in adenomas and adenocarcinomas of the colon : Its relationship with differentiation. In: Journal of Pathology. 1996 ; Vol. 179, No. 3. pp. 248-253.
@article{aaec235930a740ae9f16845a55fc6473,
title = "p21/WAF1/CIP1 expression in normal mucosa and in adenomas and adenocarcinomas of the colon: Its relationship with differentiation",
abstract = "The immunoreactivity of p21 was evaluated in normal mucosa and in adenomas and adenocarcinomas of the human large bowel. In normal mucosa, p21 immunoreactivity was seen in the superficial third of the crypts (maturation compartment) and in surface (terminally differentiated) epithelium, and was mutually exclusive with Ki67/MIB1 reactivity. These data show that p21 expression is inversely related to proliferation and directly related to terminal differentiation. In adenomas, p21-reactive cells were frequently clustered in the superficial areas and were non-reactive for MIB1. In adenocarcinomas, p21 staining was heterogeneous: high p21 expression (19 cases) was associated with lower stage and lack of p53 overexpression. p21-reactive cells were devoid of MIB1 immunoreactivity, but no relationship could be found between p21 and MIB1 labelling indices. p21 heterogeneity may be related to alterations in the p53-dependent induction pathway: high p21 expression was associated with low to absent p53 reactivity, with presumed normal p53 function; low p21 expression was associated with p53 overexpression, with presumed p53 alteration resulting in loss of function.",
keywords = "colon, cyclin-dependent inhibitor, differentiation, human, immunohistochemistry, neoplasm, p21, p53, proliferation",
author = "Claudio Doglioni and Paola Pelosio and Licia Laurino and Ettore Macri and Enzo Meggiolaro and Franco Favretti and Mattia Barbareschi",
year = "1996",
doi = "10.1002/(SICI)1096-9896(199607)179:3<248::AID-PATH571>3.0.CO;2-6",
language = "English",
volume = "179",
pages = "248--253",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - p21/WAF1/CIP1 expression in normal mucosa and in adenomas and adenocarcinomas of the colon

T2 - Its relationship with differentiation

AU - Doglioni, Claudio

AU - Pelosio, Paola

AU - Laurino, Licia

AU - Macri, Ettore

AU - Meggiolaro, Enzo

AU - Favretti, Franco

AU - Barbareschi, Mattia

PY - 1996

Y1 - 1996

N2 - The immunoreactivity of p21 was evaluated in normal mucosa and in adenomas and adenocarcinomas of the human large bowel. In normal mucosa, p21 immunoreactivity was seen in the superficial third of the crypts (maturation compartment) and in surface (terminally differentiated) epithelium, and was mutually exclusive with Ki67/MIB1 reactivity. These data show that p21 expression is inversely related to proliferation and directly related to terminal differentiation. In adenomas, p21-reactive cells were frequently clustered in the superficial areas and were non-reactive for MIB1. In adenocarcinomas, p21 staining was heterogeneous: high p21 expression (19 cases) was associated with lower stage and lack of p53 overexpression. p21-reactive cells were devoid of MIB1 immunoreactivity, but no relationship could be found between p21 and MIB1 labelling indices. p21 heterogeneity may be related to alterations in the p53-dependent induction pathway: high p21 expression was associated with low to absent p53 reactivity, with presumed normal p53 function; low p21 expression was associated with p53 overexpression, with presumed p53 alteration resulting in loss of function.

AB - The immunoreactivity of p21 was evaluated in normal mucosa and in adenomas and adenocarcinomas of the human large bowel. In normal mucosa, p21 immunoreactivity was seen in the superficial third of the crypts (maturation compartment) and in surface (terminally differentiated) epithelium, and was mutually exclusive with Ki67/MIB1 reactivity. These data show that p21 expression is inversely related to proliferation and directly related to terminal differentiation. In adenomas, p21-reactive cells were frequently clustered in the superficial areas and were non-reactive for MIB1. In adenocarcinomas, p21 staining was heterogeneous: high p21 expression (19 cases) was associated with lower stage and lack of p53 overexpression. p21-reactive cells were devoid of MIB1 immunoreactivity, but no relationship could be found between p21 and MIB1 labelling indices. p21 heterogeneity may be related to alterations in the p53-dependent induction pathway: high p21 expression was associated with low to absent p53 reactivity, with presumed normal p53 function; low p21 expression was associated with p53 overexpression, with presumed p53 alteration resulting in loss of function.

KW - colon

KW - cyclin-dependent inhibitor

KW - differentiation

KW - human

KW - immunohistochemistry

KW - neoplasm

KW - p21

KW - p53

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=0029791681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029791681&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9896(199607)179:3<248::AID-PATH571>3.0.CO;2-6

DO - 10.1002/(SICI)1096-9896(199607)179:3<248::AID-PATH571>3.0.CO;2-6

M3 - Article

VL - 179

SP - 248

EP - 253

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 3

ER -