Abstract
Emerging evidences suggest that cyclin-dependent kinase inhibitors (CKIs) can regulate cellular functions other than cell cycle progression, such as differentiation and migration. Here, we report that cytoplasmic expression of p27kip1 affects microtubule (MT) stability following cell adhesion on extracellular matrix (ECM) constituents. This p27kip1 activity is due to its ability to bind and impair the function of the MT-destabilizing protein stathmin. Accordingly, upregulation of p27kip1 or downregulation of stathmin expression results in the inhibition of mesenchymal cell motility. Moreover, high stathmin and low cytoplasmic p27kip1 expression correlate with the metastatic phenotype of human sarcomas in vivo. This study provides a functional link between proliferation and invasion of tumor cells based on diverse activities of p27kip1 in different subcellular compartments.
| Original language | English |
|---|---|
| Pages (from-to) | 51-63 |
| Number of pages | 13 |
| Journal | Cancer Cell |
| Volume | 7 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2005 |
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ASJC Scopus subject areas
- Cancer Research
- Cell Biology
- Oncology
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p27Kip1-stathmin interaction influences sarcoma cell migration and invasion. / Baldassarre, Gustavo; Belletti, Barbara; Nicoloso, Milena S.; Schiappacassi, Monica; Vecchione, Andrea; Spessotto, Paola; Morrione, Andrea; Canzonieri, Vincenzo; Colombatti, Alfonso.
In: Cancer Cell, Vol. 7, No. 1, 01.2005, p. 51-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - p27Kip1-stathmin interaction influences sarcoma cell migration and invasion
AU - Baldassarre, Gustavo
AU - Belletti, Barbara
AU - Nicoloso, Milena S.
AU - Schiappacassi, Monica
AU - Vecchione, Andrea
AU - Spessotto, Paola
AU - Morrione, Andrea
AU - Canzonieri, Vincenzo
AU - Colombatti, Alfonso
PY - 2005/1
Y1 - 2005/1
N2 - Emerging evidences suggest that cyclin-dependent kinase inhibitors (CKIs) can regulate cellular functions other than cell cycle progression, such as differentiation and migration. Here, we report that cytoplasmic expression of p27kip1 affects microtubule (MT) stability following cell adhesion on extracellular matrix (ECM) constituents. This p27kip1 activity is due to its ability to bind and impair the function of the MT-destabilizing protein stathmin. Accordingly, upregulation of p27kip1 or downregulation of stathmin expression results in the inhibition of mesenchymal cell motility. Moreover, high stathmin and low cytoplasmic p27kip1 expression correlate with the metastatic phenotype of human sarcomas in vivo. This study provides a functional link between proliferation and invasion of tumor cells based on diverse activities of p27kip1 in different subcellular compartments.
AB - Emerging evidences suggest that cyclin-dependent kinase inhibitors (CKIs) can regulate cellular functions other than cell cycle progression, such as differentiation and migration. Here, we report that cytoplasmic expression of p27kip1 affects microtubule (MT) stability following cell adhesion on extracellular matrix (ECM) constituents. This p27kip1 activity is due to its ability to bind and impair the function of the MT-destabilizing protein stathmin. Accordingly, upregulation of p27kip1 or downregulation of stathmin expression results in the inhibition of mesenchymal cell motility. Moreover, high stathmin and low cytoplasmic p27kip1 expression correlate with the metastatic phenotype of human sarcomas in vivo. This study provides a functional link between proliferation and invasion of tumor cells based on diverse activities of p27kip1 in different subcellular compartments.
UR - http://www.scopus.com/inward/record.url?scp=12344274508&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12344274508&partnerID=8YFLogxK
U2 - 10.1016/j.ccr.2004.11.025
DO - 10.1016/j.ccr.2004.11.025
M3 - Article
C2 - 15652749
AN - SCOPUS:12344274508
VL - 7
SP - 51
EP - 63
JO - Cancer Cell
JF - Cancer Cell
SN - 1535-6108
IS - 1
ER -