p27Kip1-stathmin interaction influences sarcoma cell migration and invasion

Gustavo Baldassarre, Barbara Belletti, Milena S. Nicoloso, Monica Schiappacassi, Andrea Vecchione, Paola Spessotto, Andrea Morrione, Vincenzo Canzonieri, Alfonso Colombatti

Research output: Contribution to journalArticlepeer-review

Abstract

Emerging evidences suggest that cyclin-dependent kinase inhibitors (CKIs) can regulate cellular functions other than cell cycle progression, such as differentiation and migration. Here, we report that cytoplasmic expression of p27kip1 affects microtubule (MT) stability following cell adhesion on extracellular matrix (ECM) constituents. This p27kip1 activity is due to its ability to bind and impair the function of the MT-destabilizing protein stathmin. Accordingly, upregulation of p27kip1 or downregulation of stathmin expression results in the inhibition of mesenchymal cell motility. Moreover, high stathmin and low cytoplasmic p27kip1 expression correlate with the metastatic phenotype of human sarcomas in vivo. This study provides a functional link between proliferation and invasion of tumor cells based on diverse activities of p27kip1 in different subcellular compartments.

Original languageEnglish
Pages (from-to)51-63
Number of pages13
JournalCancer Cell
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2005

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

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