P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons

Fulvio Florenzano, Maria Teresa Viscomi, Valentina Mercaldo, Patrizia Longone, Giorgio Bernardi, Claudia Bagni, Marco Molinari, Pascal Carrive

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it in the perifornical hypothalamic area, where orexinergic neurons are located. Here we report the presence of the P2X2R subunit in the rat perifornical hypothalamus and demonstrate that hypothalamic orexin neurons express the P2X2R. Double immunohistochemistry showed that virtually all orexin-immunoreactive neurons are also P2X2R immunoreactive, whereas 80% of P2X2R- immunoreactive neurons are also orexin positive. Triple-labeling experiments, combining fluorescence in situ hybridization for P2X2R mRNA and P2X2R/orexin double immunofluorescence, confirmed these findings. In addition, in situ hybridization demonstrated that P2X2R mRNA is localized in cellular processes of orexinergic neurons. The present data support neurophysiologic findings on ATP modulation of orexinergic function and provide direct evidence that the entire population of orexin neurons expresses a P2XR subtype, namely, P2X2R. Thus, purinergic transmission might intervene in modulating key functions known to be controlled by the orexinergic system, such as feeding behavior and arousal.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalJournal of Comparative Neurology
Volume498
Issue number1
DOIs
Publication statusPublished - Sep 1 2006

Fingerprint

Purinergic Receptors
Protein Subunits
Neurons
Messenger RNA
Hypothalamus
Adenosine Triphosphate
Feeding Behavior
Orexins
Arousal
Fluorescence In Situ Hybridization
In Situ Hybridization
Fluorescent Antibody Technique
Immunohistochemistry
Population

Keywords

  • ATP receptors
  • Autonomic regulation
  • Eating disorders
  • mRNA localization
  • Perifornical hypothalamus
  • Sleep-wake cycle

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons. / Florenzano, Fulvio; Viscomi, Maria Teresa; Mercaldo, Valentina; Longone, Patrizia; Bernardi, Giorgio; Bagni, Claudia; Molinari, Marco; Carrive, Pascal.

In: Journal of Comparative Neurology, Vol. 498, No. 1, 01.09.2006, p. 58-67.

Research output: Contribution to journalArticle

Florenzano, Fulvio ; Viscomi, Maria Teresa ; Mercaldo, Valentina ; Longone, Patrizia ; Bernardi, Giorgio ; Bagni, Claudia ; Molinari, Marco ; Carrive, Pascal. / P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons. In: Journal of Comparative Neurology. 2006 ; Vol. 498, No. 1. pp. 58-67.
@article{410564e0d38a4d55b614088abb97fde3,
title = "P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons",
abstract = "Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it in the perifornical hypothalamic area, where orexinergic neurons are located. Here we report the presence of the P2X2R subunit in the rat perifornical hypothalamus and demonstrate that hypothalamic orexin neurons express the P2X2R. Double immunohistochemistry showed that virtually all orexin-immunoreactive neurons are also P2X2R immunoreactive, whereas 80{\%} of P2X2R- immunoreactive neurons are also orexin positive. Triple-labeling experiments, combining fluorescence in situ hybridization for P2X2R mRNA and P2X2R/orexin double immunofluorescence, confirmed these findings. In addition, in situ hybridization demonstrated that P2X2R mRNA is localized in cellular processes of orexinergic neurons. The present data support neurophysiologic findings on ATP modulation of orexinergic function and provide direct evidence that the entire population of orexin neurons expresses a P2XR subtype, namely, P2X2R. Thus, purinergic transmission might intervene in modulating key functions known to be controlled by the orexinergic system, such as feeding behavior and arousal.",
keywords = "ATP receptors, Autonomic regulation, Eating disorders, mRNA localization, Perifornical hypothalamus, Sleep-wake cycle",
author = "Fulvio Florenzano and Viscomi, {Maria Teresa} and Valentina Mercaldo and Patrizia Longone and Giorgio Bernardi and Claudia Bagni and Marco Molinari and Pascal Carrive",
year = "2006",
month = "9",
day = "1",
doi = "10.1002/cne.21013",
language = "English",
volume = "498",
pages = "58--67",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - P2X2R purinergic receptor subunit mRNA and protein are expressed by all hypothalamic hypocretin/orexin neurons

AU - Florenzano, Fulvio

AU - Viscomi, Maria Teresa

AU - Mercaldo, Valentina

AU - Longone, Patrizia

AU - Bernardi, Giorgio

AU - Bagni, Claudia

AU - Molinari, Marco

AU - Carrive, Pascal

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it in the perifornical hypothalamic area, where orexinergic neurons are located. Here we report the presence of the P2X2R subunit in the rat perifornical hypothalamus and demonstrate that hypothalamic orexin neurons express the P2X2R. Double immunohistochemistry showed that virtually all orexin-immunoreactive neurons are also P2X2R immunoreactive, whereas 80% of P2X2R- immunoreactive neurons are also orexin positive. Triple-labeling experiments, combining fluorescence in situ hybridization for P2X2R mRNA and P2X2R/orexin double immunofluorescence, confirmed these findings. In addition, in situ hybridization demonstrated that P2X2R mRNA is localized in cellular processes of orexinergic neurons. The present data support neurophysiologic findings on ATP modulation of orexinergic function and provide direct evidence that the entire population of orexin neurons expresses a P2XR subtype, namely, P2X2R. Thus, purinergic transmission might intervene in modulating key functions known to be controlled by the orexinergic system, such as feeding behavior and arousal.

AB - Neurophysiologic data suggest that orexin neurons are directly excited by ATP through purinergic receptors (P2XR). Anatomical studies, though reporting P2XR in the hypothalamus, did not describe it in the perifornical hypothalamic area, where orexinergic neurons are located. Here we report the presence of the P2X2R subunit in the rat perifornical hypothalamus and demonstrate that hypothalamic orexin neurons express the P2X2R. Double immunohistochemistry showed that virtually all orexin-immunoreactive neurons are also P2X2R immunoreactive, whereas 80% of P2X2R- immunoreactive neurons are also orexin positive. Triple-labeling experiments, combining fluorescence in situ hybridization for P2X2R mRNA and P2X2R/orexin double immunofluorescence, confirmed these findings. In addition, in situ hybridization demonstrated that P2X2R mRNA is localized in cellular processes of orexinergic neurons. The present data support neurophysiologic findings on ATP modulation of orexinergic function and provide direct evidence that the entire population of orexin neurons expresses a P2XR subtype, namely, P2X2R. Thus, purinergic transmission might intervene in modulating key functions known to be controlled by the orexinergic system, such as feeding behavior and arousal.

KW - ATP receptors

KW - Autonomic regulation

KW - Eating disorders

KW - mRNA localization

KW - Perifornical hypothalamus

KW - Sleep-wake cycle

UR - http://www.scopus.com/inward/record.url?scp=33746866738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746866738&partnerID=8YFLogxK

U2 - 10.1002/cne.21013

DO - 10.1002/cne.21013

M3 - Article

VL - 498

SP - 58

EP - 67

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 1

ER -