P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells

Giulia Arslan, Catalin M. Filipeanu, Eva Irenius, Björn Kull, Emilio Clementi, Clemens Allgaier, David Erlinge, Bertil B. Fredholm

Research output: Contribution to journalArticle

Abstract

ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, α,β-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype. After treatment with NGF for 7 days the ATP response was increased and partially sensitive to PPADS. A component of the ATP-induced Ca2+ increase was due to mobilisation of intracellular Ca2+ stores and another to capacitative Ca2+ entry. UTP caused an increase in intracellular Ca2+, and InsP3 formation could be stimulated by ATP and UTP. ATP also caused a small increase in cyclic AMP, but this was abolished in the presence of indomethacin. Thus, after NGF treatment ATP acts partially via a P2Y receptor, possibly the P2Y2 subtype. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)482-496
Number of pages15
JournalNeuropharmacology
Volume39
Issue number3
DOIs
Publication statusPublished - Mar 2000

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Keywords

  • Cyclic AMP
  • Inositol phosphate
  • Nerve Growth Factor
  • P2X receptors
  • Purines
  • UTP

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Arslan, G., Filipeanu, C. M., Irenius, E., Kull, B., Clementi, E., Allgaier, C., Erlinge, D., & Fredholm, B. B. (2000). P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells. Neuropharmacology, 39(3), 482-496. https://doi.org/10.1016/S0028-3908(99)00141-0