P2Y1 receptor switches to neurons from glia in juvenile versus neonatal rat cerebellar cortex

Susanna Amadio, Fabrizio Vacca, Alessandro Martorana, Giuseppe Sancesario, Cinzia Volonté

Research output: Contribution to journalArticle

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Abstract

Background. In the CNS, several P2 receptors for extracellular nucleotides are identified on neurons and glial cells to participate to neuron-neuron, glia-glia and glia-neuron communication. Results. In this work, we describe the cellular and subcellular presence of metabotropic P2Y1 receptor in rat cerebellum at two distinct developmental ages, by means of immunofluorescence-confocal and electron microscopy as well as western blotting and direct membrane separation techniques. At postnatal day 21, we find that P2Y1 receptor in addition to Purkinje neurons, is abundant on neuronal specializations identified as noradrenergic by anatomical, morphological and biochemical features. P2Y1 receptor immunoreactivity colocalizes with dopamine β-hydroxylase, tyrosine hydroxylase, neurofilament light chain, synaptophysin and flotillin, but not with glial fibrillary acidic protein for astrocytes. P2Y1 receptor is found enriched in membrane microdomains such as lipid rafts, in cerebellar synaptic vesicles, and is moreover visualized on synaptic varicosities by electron microscopy analysis. When examined at postnatal day 7, P2Y1 receptor immunoreactivity is instead predominantly expressed only on Bergmann and astroglial cells, as shown by colocalization with glial fibrillary acidic protein rather then neuronal markers. At this age, we moreover identify that P2Y1 receptor-positive Bergmann fibers wrap up doublecortin-positive granule cells stretching along them, while migrating through the cerebellar layers. Conclusion. Membrane components including purinergic receptors are already known to mediate cellular contact and aggregation in platelets. Our results suggesting a potential role for P2Y1 protein in cell junction/communication and development, are totally innovative for the CNS.

Original languageEnglish
Article number77
JournalBMC Developmental Biology
Volume7
DOIs
Publication statusPublished - 2007

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Purinergic P2Y1 Receptors
Cerebellar Cortex
Neuroglia
Neurons
Glial Fibrillary Acidic Protein
Electron Microscopy
Membrane Microdomains
Purinergic Receptors
Synaptophysin
Intercellular Junctions
Membranes
Intermediate Filaments
Synaptic Vesicles
Purkinje Cells
Tyrosine 3-Monooxygenase
Mixed Function Oxygenases
Platelet Aggregation
Fluorescence Microscopy
Confocal Microscopy
Cell Communication

ASJC Scopus subject areas

  • Medicine(all)

Cite this

P2Y1 receptor switches to neurons from glia in juvenile versus neonatal rat cerebellar cortex. / Amadio, Susanna; Vacca, Fabrizio; Martorana, Alessandro; Sancesario, Giuseppe; Volonté, Cinzia.

In: BMC Developmental Biology, Vol. 7, 77, 2007.

Research output: Contribution to journalArticle

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