P2Yreceptor on the verge of a neuroinflammatory breakdown

Susanna Amadio, Chiara Parisi, Cinzia Montilli, Alberto Savio Carrubba, Savina Apolloni, Cinzia Volonté

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Ysubtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Yreceptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Yexpression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS.

Original languageEnglish
Article number975849
JournalMediators of Inflammation
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Amyotrophic Lateral Sclerosis
Microglia
Multiple Sclerosis
Oligodendroglia
Chemotaxis
Astrocytes
Cerebellum
Cues
Spinal Cord
Nucleotides
Pathology
Phenotype
Antibodies

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Medicine(all)

Cite this

P2Yreceptor on the verge of a neuroinflammatory breakdown. / Amadio, Susanna; Parisi, Chiara; Montilli, Cinzia; Carrubba, Alberto Savio; Apolloni, Savina; Volonté, Cinzia.

In: Mediators of Inflammation, Vol. 2014, 975849, 2014.

Research output: Contribution to journalArticle

Amadio, Susanna ; Parisi, Chiara ; Montilli, Cinzia ; Carrubba, Alberto Savio ; Apolloni, Savina ; Volonté, Cinzia. / P2Yreceptor on the verge of a neuroinflammatory breakdown. In: Mediators of Inflammation. 2014 ; Vol. 2014.
@article{72af26a29555421d9be504b85c20344c,
title = "P2Yreceptor on the verge of a neuroinflammatory breakdown",
abstract = "In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Ysubtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Yreceptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Yexpression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS.",
author = "Susanna Amadio and Chiara Parisi and Cinzia Montilli and Carrubba, {Alberto Savio} and Savina Apolloni and Cinzia Volont{\'e}",
year = "2014",
doi = "10.1155/2014/975849",
language = "English",
volume = "2014",
journal = "Mediators of Inflammation",
issn = "0962-9351",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - P2Yreceptor on the verge of a neuroinflammatory breakdown

AU - Amadio, Susanna

AU - Parisi, Chiara

AU - Montilli, Cinzia

AU - Carrubba, Alberto Savio

AU - Apolloni, Savina

AU - Volonté, Cinzia

PY - 2014

Y1 - 2014

N2 - In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Ysubtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Yreceptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Yexpression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS.

AB - In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Ysubtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Yreceptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Yexpression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS.

UR - http://www.scopus.com/inward/record.url?scp=84919812001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919812001&partnerID=8YFLogxK

U2 - 10.1155/2014/975849

DO - 10.1155/2014/975849

M3 - Article

VL - 2014

JO - Mediators of Inflammation

JF - Mediators of Inflammation

SN - 0962-9351

M1 - 975849

ER -