TY - JOUR
T1 - p46, a novel natural killer cell-specific surface molecule that mediates cell activation
AU - Sivori, Simona
AU - Vitale, Massimo
AU - Morelli, Luigia
AU - Sanseverino, Lorenza
AU - Augugliaro, Raffaella
AU - Bottino, Cristina
AU - Moretta, Lorenzo
AU - Moretta, Alessandro
PY - 1997/10/6
Y1 - 1997/10/6
N2 - Limited information is available on the surface molecules that are involved in natural killer (NK) cell triggering. In this study, we selected the BAB281 monoclonal antibody (mAb) on the basis of its ability to trigger NK-mediated target cell lysis. BAB281 identified a novel NK cell-specific surface molecule of 46 kD (p46) that is expressed by all resting or activated NK cells. Importantly, unlike the NK cell antigens identified so far, the expression of p46 was strictly confined to NK cells. Upon mAb-mediated cross- linking, p46 molecules induced strong cell triggering leading to [Ca2+](i) increases, lymphokine production, and cytolytic activity both in resting NK cells and NK cell clones. The p46-mediated induction of Ca2+ increases or triggering of cytolytic activity was downregulated by the simultaneous engagement of inhibitory receptors including p58, p70, and CD94/NKG2A. Both the unique cellular distribution and functional capability of p46 molecules suggest a possible role in the mechanisms of non-major histocompatibility complex-restricted cytolysis mediated by human NK cells.
AB - Limited information is available on the surface molecules that are involved in natural killer (NK) cell triggering. In this study, we selected the BAB281 monoclonal antibody (mAb) on the basis of its ability to trigger NK-mediated target cell lysis. BAB281 identified a novel NK cell-specific surface molecule of 46 kD (p46) that is expressed by all resting or activated NK cells. Importantly, unlike the NK cell antigens identified so far, the expression of p46 was strictly confined to NK cells. Upon mAb-mediated cross- linking, p46 molecules induced strong cell triggering leading to [Ca2+](i) increases, lymphokine production, and cytolytic activity both in resting NK cells and NK cell clones. The p46-mediated induction of Ca2+ increases or triggering of cytolytic activity was downregulated by the simultaneous engagement of inhibitory receptors including p58, p70, and CD94/NKG2A. Both the unique cellular distribution and functional capability of p46 molecules suggest a possible role in the mechanisms of non-major histocompatibility complex-restricted cytolysis mediated by human NK cells.
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U2 - 10.1084/jem.186.7.1129
DO - 10.1084/jem.186.7.1129
M3 - Article
C2 - 9314561
AN - SCOPUS:0030809965
VL - 186
SP - 1129
EP - 1136
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 7
ER -