p49, A putative HLA-G1-specific inhibitory NK receptor belonging to the Immunoglobulin Superfamily

Claudia Cantoni, Michela Falco, Anna Pessino, Alessandro Moretta, Lorenzo Moretta, Roberto Biassoni

Research output: Contribution to journalArticlepeer-review

Abstract

NK cells display several killer inhibitory receptors (KIRs) specific for different alleles of major histocompatibility complex (MHC) class I molecules. A family of KIRs are represented by type I transmembrane proteins belonging to the Immunoglobulin Superfamily (Ig-SF). In the present study we describe a cDNA, termed cl.15.212, that encodes for a type I transmembrane protein displaying approximately 50% sequence homology with other Ig-SF members. The protein encoded by cl.15.212 (termed p49 according to its apparent molecular weight of 49 kDa) is characterized by two extracellular Ig-like domains, a 115-amino acid cytoplasmic tail containing a single immuno-receptor tyrosine-based inhibitory motif (ITIM) typical of KIR. Different from the other KIRs, the cl.15.212 transcript is expressed by all NK cells and by a fraction of T-cell clones expressing KIR. To determine the specificity of the cl.15.212-encoded receptor, we generated a chimeric protein, formed by the ectodomain of p49 and the Fc portion of human IgG1 (p49-Fc). Soluble molecules bound efficiently to LCL721.221 (221) cells transfected with HLA-G1, -A3, -B46 alleles and weakly to the -B7 allele. On the other hand, they did not bind to 221 cells either untransfected or transfected with HLA-A2, -B51, -Cw3, or-Cw4. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalJournal of Reproductive Immunology
Volume43
Issue number2
DOIs
Publication statusPublished - Jul 1999

Keywords

  • HLA
  • KIR
  • NK cells

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

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