p53 Expression, DNA content and cell proliferation in primary and synchronous metastatic lesions from ovarian surface epithelial-stromal tumours

M. G. Daidone, E. Benini, B. Valentinis, G. Tomasic, G. Bolis, A. Villa, R. Silvestrini

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate biological heterogeneity between primary and metastatic ovarian cancer lesions from individual patients as a means of elucidating steps in clinical progression. Cancer tissue from 61 untreated patients with ovarian surface epithelial-stromal tumours was examined. p53 expression detected immunocytochemically by the PAb1801 antibody, DNA content evaluated by flow cytometry, and cell proliferation evaluated as the [3H]thymidine labelling index were investigated in primary tumours and corresponding synchronous metastases. The frequency of p53 positivity was similar in primary (62%) and metastatic (66%) sites, with an agreement between the two lesions from the same patient in 97% of the cases. Similarly, aneuploidy frequency (80%) and DNA indices were superimposable in primary and metastatic lesions from the same patient, with a 94% agreement; The frequency of aneuploidy was higher in p53-positive than in p53-negative lesions. An overall poor agreement (r(s) = 0.44) was observed for proliferative activity of primary and metastatic lesions, due to a heterogeneous profile in omental with respect to primary tumours, which was mainly evident in p53-positive cancers. Conversely, cell proliferation of peritoneal, abdominal and pelvic lesions was qualitatively similar to that of the primary tumour in 88% of patients.

Original languageEnglish
Pages (from-to)1388-1393
Number of pages6
JournalEuropean Journal of Cancer
Volume32
Issue number8
DOIs
Publication statusPublished - 1996

Keywords

  • Interlesional heterogeneity
  • Ovarian surface epithelial-stromal tumour
  • P53
  • Ploidy
  • Proliferative activity
  • Synchronous lesions

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Fingerprint Dive into the research topics of 'p53 Expression, DNA content and cell proliferation in primary and synchronous metastatic lesions from ovarian surface epithelial-stromal tumours'. Together they form a unique fingerprint.

  • Cite this