p53 Mutations are associated with histologic transformation of follicular lymphoma

F. Lo Coco, G. Gaidano, D. C. Louie, K. Offit, R. S K Chaganti, R. Dalla-Favera

Research output: Contribution to journalArticle

Abstract

The majority of low-grade non-Hodgkin's lymphomas (NHL) undergo clinical progression toward intermediate- and high-grade lymphomas. This progression is often associated with histologic transformation from follicular to diffuse-type NHL. The pathogenetic mechanisms underlying this evolution are presently unknown. In this study, we have analyzed the role in NHL progression of relevant genetic lesions affecting proto-oncogenes and tumor suppressor genes. Sequential biopsies from 21 patients with clinical progression with (5 cases) or without (16 cases) evidence of histologic transformation were analyzed for karyotypic changes, c-myc rearrangements and deletions affecting 6q27 by Southern blot analysis, and p53 mutations by single-strand conformation polymorphism (SSCP) analysis coupled with direct sequencing of polymerase chain reaction-amplified products. No novel cytogenetic aberration was detected in association with progression, and all samples analyzed displayed a normal c-myc gene. Mutations of the p53 gene were detected in 4 of 5 cases displaying histologic transformation from follicular to diffuse-type NHL and in none of the 16 cases displaying clinical progression in the absence of histologic transformation. In 1 of these positive cases, the same mutation was also present in the pretransformation biopsy, correlating with the presence of diffuse-type areas within a predominant follicular pattern. In 1 of these cases, a deletion of 6q27 was also detected in the posttransformation biopsy along with a p53 mutation. These findings indicate that p53 mutations are associated with and may be responsible for histologic transformation of follicular lymphoma.

Original languageEnglish
Pages (from-to)2289-2295
Number of pages7
JournalBlood
Volume82
Issue number8
Publication statusPublished - 1993

Fingerprint

Follicular Lymphoma
Biopsy
Non-Hodgkin's Lymphoma
Genes
Mutation
Polymerase chain reaction
Polymorphism
Aberrations
Conformations
Tumors
Association reactions
myc Genes
Proto-Oncogenes
p53 Genes
Southern Blotting
Tumor Suppressor Genes
Chromosome Aberrations
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Hematology

Cite this

Lo Coco, F., Gaidano, G., Louie, D. C., Offit, K., Chaganti, R. S. K., & Dalla-Favera, R. (1993). p53 Mutations are associated with histologic transformation of follicular lymphoma. Blood, 82(8), 2289-2295.

p53 Mutations are associated with histologic transformation of follicular lymphoma. / Lo Coco, F.; Gaidano, G.; Louie, D. C.; Offit, K.; Chaganti, R. S K; Dalla-Favera, R.

In: Blood, Vol. 82, No. 8, 1993, p. 2289-2295.

Research output: Contribution to journalArticle

Lo Coco, F, Gaidano, G, Louie, DC, Offit, K, Chaganti, RSK & Dalla-Favera, R 1993, 'p53 Mutations are associated with histologic transformation of follicular lymphoma', Blood, vol. 82, no. 8, pp. 2289-2295.
Lo Coco F, Gaidano G, Louie DC, Offit K, Chaganti RSK, Dalla-Favera R. p53 Mutations are associated with histologic transformation of follicular lymphoma. Blood. 1993;82(8):2289-2295.
Lo Coco, F. ; Gaidano, G. ; Louie, D. C. ; Offit, K. ; Chaganti, R. S K ; Dalla-Favera, R. / p53 Mutations are associated with histologic transformation of follicular lymphoma. In: Blood. 1993 ; Vol. 82, No. 8. pp. 2289-2295.
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