p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder: Correlation with histological parameters

Patrizia Tenti, Rita Zappatore, Solange Romagnoli, Emilio Civardi, Paolo Giunta, Roberto Scelsi, Giorgio Stella, Luciano Carnevali

Research output: Contribution to journalArticle

Abstract

Seventy-nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40.5 per cent of the cases; the percentage of positive cases was significantly lower in low-grade (G1 and G2) TCCs than in high-grade (G3) tumours (10.6 per cent vs. 84.4 per cent; P <0.0001). The overall rate of HPV infection was 32.9 per cent; 20.3 per cent of the cases were positive for HPV 16, 3.8 per cent for HPV 18, and 8.9 per cent for both. Consensus primers as well as type-specific primers for HPV tvpes 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low-grade than in high-grade tumours (44.7 per cent vs. 15.6 per cent; P = 0.0061). p53-positive cases were more common among papillary, deeply infiltrating tumours, and HPV-positive cases among papillary, non-infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low-grade and in invasive high-grade tumours is suggested.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalJournal of Pathology
Volume178
Issue number1
DOIs
Publication statusPublished - 1996

Fingerprint

Papillomavirus Infections
Transitional Cell Carcinoma
Urinary Bladder
Human papillomavirus 18
Human papillomavirus 16
Neoplasms
Human papillomavirus 6
Immunohistochemistry
Monoclonal Antibodies
Polymerase Chain Reaction
Infection

Keywords

  • HPV
  • p53
  • Transitional cell carcinoma
  • Urinary bladder

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder : Correlation with histological parameters. / Tenti, Patrizia; Zappatore, Rita; Romagnoli, Solange; Civardi, Emilio; Giunta, Paolo; Scelsi, Roberto; Stella, Giorgio; Carnevali, Luciano.

In: Journal of Pathology, Vol. 178, No. 1, 1996, p. 65-70.

Research output: Contribution to journalArticle

Tenti, Patrizia ; Zappatore, Rita ; Romagnoli, Solange ; Civardi, Emilio ; Giunta, Paolo ; Scelsi, Roberto ; Stella, Giorgio ; Carnevali, Luciano. / p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder : Correlation with histological parameters. In: Journal of Pathology. 1996 ; Vol. 178, No. 1. pp. 65-70.
@article{18efc5ebb5b2423eb3a079ec33af3ce3,
title = "p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder: Correlation with histological parameters",
abstract = "Seventy-nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40.5 per cent of the cases; the percentage of positive cases was significantly lower in low-grade (G1 and G2) TCCs than in high-grade (G3) tumours (10.6 per cent vs. 84.4 per cent; P <0.0001). The overall rate of HPV infection was 32.9 per cent; 20.3 per cent of the cases were positive for HPV 16, 3.8 per cent for HPV 18, and 8.9 per cent for both. Consensus primers as well as type-specific primers for HPV tvpes 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low-grade than in high-grade tumours (44.7 per cent vs. 15.6 per cent; P = 0.0061). p53-positive cases were more common among papillary, deeply infiltrating tumours, and HPV-positive cases among papillary, non-infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low-grade and in invasive high-grade tumours is suggested.",
keywords = "HPV, p53, Transitional cell carcinoma, Urinary bladder",
author = "Patrizia Tenti and Rita Zappatore and Solange Romagnoli and Emilio Civardi and Paolo Giunta and Roberto Scelsi and Giorgio Stella and Luciano Carnevali",
year = "1996",
doi = "10.1002/(SICI)1096-9896(199601)178:1<65::AID-PATH451>3.0.CO;2-W",
language = "English",
volume = "178",
pages = "65--70",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder

T2 - Correlation with histological parameters

AU - Tenti, Patrizia

AU - Zappatore, Rita

AU - Romagnoli, Solange

AU - Civardi, Emilio

AU - Giunta, Paolo

AU - Scelsi, Roberto

AU - Stella, Giorgio

AU - Carnevali, Luciano

PY - 1996

Y1 - 1996

N2 - Seventy-nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40.5 per cent of the cases; the percentage of positive cases was significantly lower in low-grade (G1 and G2) TCCs than in high-grade (G3) tumours (10.6 per cent vs. 84.4 per cent; P <0.0001). The overall rate of HPV infection was 32.9 per cent; 20.3 per cent of the cases were positive for HPV 16, 3.8 per cent for HPV 18, and 8.9 per cent for both. Consensus primers as well as type-specific primers for HPV tvpes 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low-grade than in high-grade tumours (44.7 per cent vs. 15.6 per cent; P = 0.0061). p53-positive cases were more common among papillary, deeply infiltrating tumours, and HPV-positive cases among papillary, non-infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low-grade and in invasive high-grade tumours is suggested.

AB - Seventy-nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40.5 per cent of the cases; the percentage of positive cases was significantly lower in low-grade (G1 and G2) TCCs than in high-grade (G3) tumours (10.6 per cent vs. 84.4 per cent; P <0.0001). The overall rate of HPV infection was 32.9 per cent; 20.3 per cent of the cases were positive for HPV 16, 3.8 per cent for HPV 18, and 8.9 per cent for both. Consensus primers as well as type-specific primers for HPV tvpes 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low-grade than in high-grade tumours (44.7 per cent vs. 15.6 per cent; P = 0.0061). p53-positive cases were more common among papillary, deeply infiltrating tumours, and HPV-positive cases among papillary, non-infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low-grade and in invasive high-grade tumours is suggested.

KW - HPV

KW - p53

KW - Transitional cell carcinoma

KW - Urinary bladder

UR - http://www.scopus.com/inward/record.url?scp=0030069702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030069702&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9896(199601)178:1<65::AID-PATH451>3.0.CO;2-W

DO - 10.1002/(SICI)1096-9896(199601)178:1<65::AID-PATH451>3.0.CO;2-W

M3 - Article

C2 - 8778319

AN - SCOPUS:0030069702

VL - 178

SP - 65

EP - 70

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 1

ER -