p53 overexpression and human papillomavirus infection in transitional cell carcinoma of the urinary bladder: Correlation with histological parameters

Patrizia Tenti, Rita Zappatore, Solange Romagnoli, Emilio Civardi, Paolo Giunta, Roberto Scelsi, Giorgio Stella, Luciano Carnevali

Research output: Contribution to journalArticlepeer-review

Abstract

Seventy-nine transitional cell carcinomas (TCCs) of the urinary bladder (25 grade 1, 22 grade 2, and 32 grade 3 tumours) were examined for p53 overexpression by immunohistochemistry with a monoclonal antibody and for human papillomavirus (HPV) infection by the polymerase chain reaction (PCR). Positive immunostaining for p53 was detected in 40.5 per cent of the cases; the percentage of positive cases was significantly lower in low-grade (G1 and G2) TCCs than in high-grade (G3) tumours (10.6 per cent vs. 84.4 per cent; P <0.0001). The overall rate of HPV infection was 32.9 per cent; 20.3 per cent of the cases were positive for HPV 16, 3.8 per cent for HPV 18, and 8.9 per cent for both. Consensus primers as well as type-specific primers for HPV tvpes 6, 11, and 33 failed to detect any additional case with HPV infection. The prevalence of HPV 16 and/or HPV 18 infection was significantly higher in low-grade than in high-grade tumours (44.7 per cent vs. 15.6 per cent; P = 0.0061). p53-positive cases were more common among papillary, deeply infiltrating tumours, and HPV-positive cases among papillary, non-infiltrating lesions. According to these data, p53 overexpression and HPV 16/18 infection are common findings in bladder TCC and there appears to be an inverse correlation of p53 overexpression and of HPV infection with tumour aggressiveness. The possibility of different molecular pathways in superficial low-grade and in invasive high-grade tumours is suggested.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalJournal of Pathology
Volume178
Issue number1
DOIs
Publication statusPublished - 1996

Keywords

  • HPV
  • p53
  • Transitional cell carcinoma
  • Urinary bladder

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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