p66Shc gene has a pro-apoptotic role in human cell lines and it is activated by a p53-independent pathway

Luca Tiberi, Amir Faisal, Matteo Rossi, Lucia Di Tella, Claudio Franceschi, Stefano Salvioli

Research output: Contribution to journalArticlepeer-review

Abstract

p66Shc protein has been proposed to be an indispensable factor for p53-dependent, mitochondria-mediated apoptosis in mice. Here, we show that p66Shc plays a pro-apoptotic role also in cell lines of human origin such as SaOs-2 and HeLa, where p53 is either absent or inactivated, thus, suggesting that p66Shc pro-apoptotic role is independent from the presence of a functional form of p53. The active form of p66Shc is phosphorylated in Serine 36. We confirm the importance of Serine 36 phosphorylation for p66Shc pro-apoptotic role, and our results suggest that the kinase involved in this process is activated independently from p53.

Original languageEnglish
Pages (from-to)503-508
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number2
DOIs
Publication statusPublished - Apr 7 2006

Keywords

  • Apoptosis
  • Human cell lines
  • Oxidative stress
  • p66
  • Serine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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