p66shc is highly expressed in fibroblasts from centenarians

S. Pandolfi, M. Bonafè, L. Di Tella, L. Tiberi, S. Salvioli, D. Monti, S. Sorbi, C. Franceschi

Research output: Contribution to journalArticle

Abstract

p66shc-/- mice exhibit prolonged lifespan and increased resistance to oxidative and hypoxic stress. To investigate p66shc involvement in human longevity, p66shc mRNA and protein were evaluated in fibroblasts from young people, elderly and centenarians, exposed to oxidative or hypoxic stress. Unexpectedly, centenarians showed the highest basal levels of p66shc. Oxidative stress induced p66shc in all samples. At variance, hypoxic stress caused p66shc reduction only in cells from centenarians. These changes occurred in absence of any modification of p66shc promoter methylation pattern. Intriguingly, in cells from centenarians, p66shc induction was affected by p53 codon 72 polymorphism. Thus, cells from centenarians present a peculiar regulation of p66shc, suggesting that its role in mammalian longevity is more complex than previously thought.

Original languageEnglish
Pages (from-to)839-844
Number of pages6
JournalMechanisms of Ageing and Development
Volume126
Issue number8
DOIs
Publication statusPublished - Aug 2005

Keywords

  • 2-Deoxy-D-ribose
  • Deferoxamine
  • DNA methylation
  • Fibroblasts
  • Longevity
  • p66

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Fingerprint Dive into the research topics of 'p66shc is highly expressed in fibroblasts from centenarians'. Together they form a unique fingerprint.

  • Cite this

    Pandolfi, S., Bonafè, M., Di Tella, L., Tiberi, L., Salvioli, S., Monti, D., Sorbi, S., & Franceschi, C. (2005). p66shc is highly expressed in fibroblasts from centenarians. Mechanisms of Ageing and Development, 126(8), 839-844. https://doi.org/10.1016/j.mad.2005.03.004