p73 overexpression increases VEGF and reduces thrombospondin-1 production: Implications for tumor angiogenesis

Faina Vikhanskaya, Maria R. Bani, Patrizia Borsotti, Carmen Ghilardi, Roberta Ceruti, Gabriele Ghisleni, Mirko Marabese, Raffaella Giavazzi, Massimo Broggini, Giulia Taraboletti

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor neovascularization is controlled by a balance between positive and negative effectors, whose production can be regulated by oncogenes and tumor suppressor genes. The aim of this study was to investigate whether the angiogenic potential of tumors could also be controlled by p73, a gene homologous to the tumor suppressor p53, whose involvement in tumor angiogenesis is known. We have studied the production of proangiogenic (VEGF, FGF-2, PIGF and PDGF) and antiangiogenic (TSP-1) factors in two p73 overexpressing clones obtained from the human ovarian carcinoma cells A2780. TSP-1 was downregulated in both clones compared to mock transfected cells, both at mRNA and protein level. Conversely, both clones showed an increased production of VEGF mRNA and protein. For both TSP-1 and VEGF, regulation of expression was partially due to modulation of the promoter activity, and was dependent on p53 status. Production of the other angiogenic factors FGF-2, PIGF and PDGF-B was also increased in p73 overexpressing clones. The two clones were more angiogenic than parental cells, as shown in vitro by their increased chemotactic activity for endothelial cells, and in vivo by the generation of more vascularized tumors. These findings suggest a potential role of p73 in tumor angiogenesis.

Original languageEnglish
Pages (from-to)7293-7300
Number of pages8
JournalOncogene
Volume20
Issue number50
DOIs
Publication statusPublished - Nov 1 2001

Keywords

  • Angiogenesis
  • Ovarian carcinoma
  • p73
  • Thrombospondin-1
  • VEGF

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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