p95-APP1 links membrane transport to Rac-mediated reorganization of actin

Alessandra Di Cesare, Simona Paris, Chiara Albertinazzi, Simona Dariozzi, Jens Andersen, Matthias Mann, Renato Longhi, Ivan De Curtis

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Motility requires protrusive activity at the cellular edge, where Rho family members regulate actin dynamics. Here we show that p95-APP1 (ArfGAP-putative, Pix-interacting, paxillin-interacting protein 1), a member of the GIT1/PKL family, is part of a complex that interacts with Rac. Wild-type and truncated p95-APP1 induce actin-rich protrusions mediated by Rac and ADP-ribosylation factor 6 (Arf6). Distinct p95-APP1-derived polypeptides have different distributions, indicating that p95-APP1 cycles between the cell surface and endosomes. Our results show that p95-APP1 functionally interacts with Rac and localizes to endosomal compartments, thus identifying p95-APP1 as a molecular link between actin organization, adhesion, and membrane transport during cell motility.

Original languageEnglish
Pages (from-to)521-530
Number of pages10
JournalNature Cell Biology
Volume2
Issue number8
DOIs
Publication statusPublished - Aug 2000

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Actins
Membranes
Paxillin
Endosomes
Cell Movement
Cell Cycle
Peptides
Proteins
ADP-ribosylation factor 6

ASJC Scopus subject areas

  • Cell Biology

Cite this

Di Cesare, A., Paris, S., Albertinazzi, C., Dariozzi, S., Andersen, J., Mann, M., ... De Curtis, I. (2000). p95-APP1 links membrane transport to Rac-mediated reorganization of actin. Nature Cell Biology, 2(8), 521-530. https://doi.org/10.1038/35019561

p95-APP1 links membrane transport to Rac-mediated reorganization of actin. / Di Cesare, Alessandra; Paris, Simona; Albertinazzi, Chiara; Dariozzi, Simona; Andersen, Jens; Mann, Matthias; Longhi, Renato; De Curtis, Ivan.

In: Nature Cell Biology, Vol. 2, No. 8, 08.2000, p. 521-530.

Research output: Contribution to journalArticle

Di Cesare, A, Paris, S, Albertinazzi, C, Dariozzi, S, Andersen, J, Mann, M, Longhi, R & De Curtis, I 2000, 'p95-APP1 links membrane transport to Rac-mediated reorganization of actin', Nature Cell Biology, vol. 2, no. 8, pp. 521-530. https://doi.org/10.1038/35019561
Di Cesare A, Paris S, Albertinazzi C, Dariozzi S, Andersen J, Mann M et al. p95-APP1 links membrane transport to Rac-mediated reorganization of actin. Nature Cell Biology. 2000 Aug;2(8):521-530. https://doi.org/10.1038/35019561
Di Cesare, Alessandra ; Paris, Simona ; Albertinazzi, Chiara ; Dariozzi, Simona ; Andersen, Jens ; Mann, Matthias ; Longhi, Renato ; De Curtis, Ivan. / p95-APP1 links membrane transport to Rac-mediated reorganization of actin. In: Nature Cell Biology. 2000 ; Vol. 2, No. 8. pp. 521-530.
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