p95-APP1 links membrane transport to Rac-mediated reorganization of actin

Alessandra Di Cesare, Simona Paris, Chiara Albertinazzi, Simona Dariozzi, Jens Andersen, Matthias Mann, Renato Longhi, Ivan De Curtis

Research output: Contribution to journalArticle


Motility requires protrusive activity at the cellular edge, where Rho family members regulate actin dynamics. Here we show that p95-APP1 (ArfGAP-putative, Pix-interacting, paxillin-interacting protein 1), a member of the GIT1/PKL family, is part of a complex that interacts with Rac. Wild-type and truncated p95-APP1 induce actin-rich protrusions mediated by Rac and ADP-ribosylation factor 6 (Arf6). Distinct p95-APP1-derived polypeptides have different distributions, indicating that p95-APP1 cycles between the cell surface and endosomes. Our results show that p95-APP1 functionally interacts with Rac and localizes to endosomal compartments, thus identifying p95-APP1 as a molecular link between actin organization, adhesion, and membrane transport during cell motility.

Original languageEnglish
Pages (from-to)521-530
Number of pages10
JournalNature Cell Biology
Issue number8
Publication statusPublished - Aug 2000

ASJC Scopus subject areas

  • Cell Biology

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    Di Cesare, A., Paris, S., Albertinazzi, C., Dariozzi, S., Andersen, J., Mann, M., Longhi, R., & De Curtis, I. (2000). p95-APP1 links membrane transport to Rac-mediated reorganization of actin. Nature Cell Biology, 2(8), 521-530. https://doi.org/10.1038/35019561