Abstract
BACKGROUND. PACAP is a member of the VIP/GHRH family of neuropeptides and has important effects on prostate cell proliferation. Here we analyze the expression and localization of PACAP and its specific receptor variants (PAC1-R) in tissues collected from patients undergoing prostate biopsy and surgery for benign prostatic hyperplasia (BPH) and prostate cancer (PCa). METHODS. Reverse transcriptase (RT)-polymerase chain reaction (PCR), DNA sequencing, and immunohistochemistry. RESULTS. PACAP and PAC1-R were localized by immunohistochemistry in the prostate tissue. While in healthy and BPH tissues PAC1-R positive staining is present in all the epithelial cells lining the lumen of the acini and in some stromal cells (mostly in the apical portion of the cells), in PCa tissues, anti-PAC1-R antibody stained the apical portion of the cells. We provide evidence that PAC 1-R null and SV1/SV2 variants are all present in normal and hyperplastic tissues, while in PCa tissue PAC1-R null is the most relevant receptor variant expressed. CONCLUSIONS. Our data demonstrates that the PAC1-R null variant is the most relevant isoform expressed in human PCa tissue being suggestively related with the events determining the outcome of prostate cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 514-521 |
| Number of pages | 8 |
| Journal | Prostate |
| Volume | 66 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Apr 1 2006 |
Fingerprint
Keywords
- NE differentiation
- PAC-R
- PACAP
- Prostate cancer
- SV
- SV
ASJC Scopus subject areas
- Urology
Cite this
PAC1-R null isoform expression in human prostate cancer tissue. / Mammi, Caterina; Frajese, Giovanni V.; Vespasiani, Giuseppe; Mariani, Stefania; Gnessi, Lucio; Farini, Donatella; Fabbri, Andrea; Frajese, Gaetano; Moretti, Costanzo.
In: Prostate, Vol. 66, No. 5, 01.04.2006, p. 514-521.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PAC1-R null isoform expression in human prostate cancer tissue
AU - Mammi, Caterina
AU - Frajese, Giovanni V.
AU - Vespasiani, Giuseppe
AU - Mariani, Stefania
AU - Gnessi, Lucio
AU - Farini, Donatella
AU - Fabbri, Andrea
AU - Frajese, Gaetano
AU - Moretti, Costanzo
PY - 2006/4/1
Y1 - 2006/4/1
N2 - BACKGROUND. PACAP is a member of the VIP/GHRH family of neuropeptides and has important effects on prostate cell proliferation. Here we analyze the expression and localization of PACAP and its specific receptor variants (PAC1-R) in tissues collected from patients undergoing prostate biopsy and surgery for benign prostatic hyperplasia (BPH) and prostate cancer (PCa). METHODS. Reverse transcriptase (RT)-polymerase chain reaction (PCR), DNA sequencing, and immunohistochemistry. RESULTS. PACAP and PAC1-R were localized by immunohistochemistry in the prostate tissue. While in healthy and BPH tissues PAC1-R positive staining is present in all the epithelial cells lining the lumen of the acini and in some stromal cells (mostly in the apical portion of the cells), in PCa tissues, anti-PAC1-R antibody stained the apical portion of the cells. We provide evidence that PAC 1-R null and SV1/SV2 variants are all present in normal and hyperplastic tissues, while in PCa tissue PAC1-R null is the most relevant receptor variant expressed. CONCLUSIONS. Our data demonstrates that the PAC1-R null variant is the most relevant isoform expressed in human PCa tissue being suggestively related with the events determining the outcome of prostate cancer.
AB - BACKGROUND. PACAP is a member of the VIP/GHRH family of neuropeptides and has important effects on prostate cell proliferation. Here we analyze the expression and localization of PACAP and its specific receptor variants (PAC1-R) in tissues collected from patients undergoing prostate biopsy and surgery for benign prostatic hyperplasia (BPH) and prostate cancer (PCa). METHODS. Reverse transcriptase (RT)-polymerase chain reaction (PCR), DNA sequencing, and immunohistochemistry. RESULTS. PACAP and PAC1-R were localized by immunohistochemistry in the prostate tissue. While in healthy and BPH tissues PAC1-R positive staining is present in all the epithelial cells lining the lumen of the acini and in some stromal cells (mostly in the apical portion of the cells), in PCa tissues, anti-PAC1-R antibody stained the apical portion of the cells. We provide evidence that PAC 1-R null and SV1/SV2 variants are all present in normal and hyperplastic tissues, while in PCa tissue PAC1-R null is the most relevant receptor variant expressed. CONCLUSIONS. Our data demonstrates that the PAC1-R null variant is the most relevant isoform expressed in human PCa tissue being suggestively related with the events determining the outcome of prostate cancer.
KW - NE differentiation
KW - PAC-R
KW - PACAP
KW - Prostate cancer
KW - SV
KW - SV
UR - http://www.scopus.com/inward/record.url?scp=33645342213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645342213&partnerID=8YFLogxK
U2 - 10.1002/pros.20356
DO - 10.1002/pros.20356
M3 - Article
C2 - 16372333
AN - SCOPUS:33645342213
VL - 66
SP - 514
EP - 521
JO - Prostate
JF - Prostate
SN - 0270-4137
IS - 5
ER -