Paclitaxel-releasing mesenchymal stromal cells inhibit in vitro proliferation of human mesothelioma cells

Francesco Petrella, Valentina Coccè, Carla Masia, Martina Milani, Emanuela Omodeo Salè, Giulio Alessandri, Eugenio Parati, Francesca Sisto, Francesca Pentimalli, Anna T. Brini, Augusto Pessina, Lorenzo Spaggiari

Research output: Contribution to journalArticle

Abstract

Introduction Malignant pleural mesothelioma (MPM) is a rare fatal asbestos-related malignancy originating in the mesothelial cells of the pleura. A platinum-based doublet containing a third-generation antifolate is the front-line standard of care whilst there are no approved second-line treatments for MPM which remains a disease setting to test the efficacy of new therapeutic agents. Methods Bone marrow mesenchymal stromal cells (BM-MSCs) were loaded with pemetrexed (PMX) and paclitaxel (PTX) according to a standardized procedure. Drug release by both PMX- and PTX-primed BM-MSCs (BM-MSCs/PMX and BM-MSCs/PTX) was tested on the in vitro proliferation of a panel of tumor cell lines including NCI-H28 mesothelioma. Results The in vitro anticancer activity of pure PTX was significantly higher than that of PMX against all the cell lines tested (14.7 times higher than that of PMX against NCI-H28). Whereas BM-MSCs did not take up and release PMX in amounts effective on mesothelioma, PTX-loaded BM-MSCs dramatically inhibited mesothelioma proliferation. Conclusions PTX-primed mesenchymal stromal cells successfully inhibit the in vitro proliferation of human mesothelioma cells. Further studies and in vivo testing are required to confirm our preliminary in vitro results as a potential new mesothelioma therapy based on cell drug delivery.

Original languageEnglish
Pages (from-to)755-758
Number of pages4
JournalBiomedicine and Pharmacotherapy
Volume87
DOIs
Publication statusPublished - Mar 1 2017

Keywords

  • Cell drug delivery
  • Mesenchymal stromal cells
  • Mesothelioma
  • Paclitaxel
  • Pemetrexed

ASJC Scopus subject areas

  • Pharmacology

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    Petrella, F., Coccè, V., Masia, C., Milani, M., Salè, E. O., Alessandri, G., Parati, E., Sisto, F., Pentimalli, F., Brini, A. T., Pessina, A., & Spaggiari, L. (2017). Paclitaxel-releasing mesenchymal stromal cells inhibit in vitro proliferation of human mesothelioma cells. Biomedicine and Pharmacotherapy, 87, 755-758. https://doi.org/10.1016/j.biopha.2017.01.118