Paclitaxel-releasing mesenchymal stromal cells inhibit the growth of multiple myeloma cells in a dynamic 3D culture system

Arianna Bonomi, Nathalie Steimberg, Anna Benetti, Angiola Berenzi, Giulio Alessandri, Luisa Pascucci, Jennifer Boniotti, Valentina Coccè, Valeria Sordi, A. Pessina, Giovanna Mazzoleni

Research output: Contribution to journalArticle

Abstract

Multiple myeloma is an aggressive tumour able to suppress osteoblastogenesis probably mediated by bone marrow mesenchymal stromal cells (BM-MSCs) that can also support plasma cell growth/survival. The use of MSCs for multiple myeloma therapy is a controversial topic because of the contradictory results on the capacity of MSCs to inhibit or to promote cancer growth. Our previous studies demonstrated that MSCs could be loaded with Paclitaxel (PTX) and used to deliver the drug in situ in amount affecting tumour growth (in vitro and in vivo). Therefore, independently on the discussed action of MSCs in myeloma, MSCs could represent a 'trojan horse' to vehicle and deliver anti-tumour agents into bone marrow. This study confirms, by an in vitro 3D dynamic culture system, that PTX loaded BM-MSCs (PTXr-MSCs) are active on the proliferation of RPMI 8226, a human myeloma cell line. Our results demonstrated a dramatic suppression of myeloma cell growth by PTXr-MSCs, suggesting that drug loaded MSCs could be a tool to deliver drug into the bone marrow. Drug releasing MSCs provide a therapeutic approach to potentiate the existing treatments against a very aggressive malignancy as multiple myeloma.

Original languageEnglish
JournalHematological Oncology
DOIs
Publication statusAccepted/In press - 2016

Keywords

  • Mesenchymal stromal cells (MSCs)
  • Multiple myeloma (MM)
  • Paclitaxel
  • Rotary cell culture system (RCCS) bioreactor
  • RPMI 8226 cells

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Bonomi, A., Steimberg, N., Benetti, A., Berenzi, A., Alessandri, G., Pascucci, L., Boniotti, J., Coccè, V., Sordi, V., Pessina, A., & Mazzoleni, G. (Accepted/In press). Paclitaxel-releasing mesenchymal stromal cells inhibit the growth of multiple myeloma cells in a dynamic 3D culture system. Hematological Oncology. https://doi.org/10.1002/hon.2306