Pain related channels are differentially expressed in neuronal and non-neuronal cells of glabrous skin of fabry knockout male mice

Jarmila Lakomá, Roberto Rimondini, Vincenzo Donadio, Rocco Liguori, Marco Caprini

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Fabry disease (FD) is one of the X-linked lysosomal storage disorders caused by deficient functioning of the alpha-galactosidase A (α-GalA) enzyme. The α-GalA deficiency leads to multi-systemic clinical manifestations caused by the preferential accumulation of globotriaosylceramide in the endothelium and vascular smooth muscles. A hallmark symptom of FD patients is peripheral pain that appears in the early stage of the disease. Pain in FD patients is a peripheral small-fiber idiopathic neuropathy, with intra-epidermal fiber density and integrity being used for diagnosing FD in humans. However, the molecular correlates underlying pain sensation in FD remain elusive. Here, we have employed the α-GalA gene KO mouse as a model of FD in rodents to investigate molecular changes in their peripheral nervous system that may account for their algesic symptoms. The α-GalA null mice display neuropathic pain as evidenced by thermal hyperalgesia and mechanical allodynia, with histological analyses showing alterations in cutaneous innervation. Additionally, KO mice showed a decreased and scattered pattern of neuronal terminations consistent with the reduction in neuronal terminations in skin biopsies of patients with small fiber neuropathies. At the molecular level KO animals showed an increase in the expression of TRPV1 and Nav1.8, and a decrease in the expression of TRPM8. Notably, these alterations are observed in young animals. Taken together, our findings imply that the α-GalA KO mouse is a good model in which to study the peripheral small fiber neuropathy exhibited by FD patients, and provides molecular evidence for a hyperexcitability of small nociceptors in FD.

Original languageEnglish
Article numbere108641
JournalPLoS One
Volume9
Issue number10
DOIs
Publication statusPublished - Oct 22 2014

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Fabry Disease
skin (animal)
Knockout Mice
peripheral nervous system diseases
pain
Skin
Pain
mice
signs and symptoms (animals and humans)
Hyperalgesia
cells
alpha-galactosidase
peripheral nervous system
disease models
young animals
innervation
Fibers
endothelium
human diseases
blood vessels

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pain related channels are differentially expressed in neuronal and non-neuronal cells of glabrous skin of fabry knockout male mice. / Lakomá, Jarmila; Rimondini, Roberto; Donadio, Vincenzo; Liguori, Rocco; Caprini, Marco.

In: PLoS One, Vol. 9, No. 10, e108641, 22.10.2014.

Research output: Contribution to journalArticle

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