Palmitoylation-dependent estrogen receptor α membrane localization: Regulation by 17β-estradiol

Filippo Acconcia, Paolo Ascenzi, Alessio Bocedi, Enzo Spisni, Vittorio Tomasi, Anna Trentalance, Paolo Visca, Maria Marino

Research output: Contribution to journalArticle

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Abstract

A fraction of the nuclear estrogen receptor α (ERα) is localized to the plasma membrane region of 17β-estradiol (E2) target cells. We previously reported that ERα is a palmitoylated protein. To gain insight into the molecular mechanism of ERα residence at the plasma membrane, we tested both the role of palmitoylation and the impact of E2 stimulation on ERα membrane localization. The cancer cell lines expressing transfected or endogenous human ERα (HeLa and HepG2, respectively) or the ERα nonpalmitoylable Cys447Ala mutant transfected in HeLa cells were used as experimental models. We found that palmitoylation of ERα enacts ERα association with the plasma membrane, interaction with the membrane protein caveolin-1, and nongenomic activities, including activation of signaling pathways and cell proliferation (i.e., ERK and AKT activation, cyclin D 1 promoter activity, DNA synthesis). Moreover, E2 reduces both ERα palmitoylation and its interaction with caveolin-1, in a time- and dose-dependent manner. These data point to the physiological role of ERα palmitoylation in the receptor localization to the cell membrane and in the regulation of the E2-induced cell proliferation.

Original languageEnglish
Pages (from-to)231-237
Number of pages7
JournalMolecular Biology of the Cell
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2005

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Lipoylation
Estrogen Receptors
Estradiol
Membranes
Cell Membrane
Caveolin 1
Cell Proliferation
Cyclin D
HeLa Cells
Membrane Proteins
Theoretical Models

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Acconcia, F., Ascenzi, P., Bocedi, A., Spisni, E., Tomasi, V., Trentalance, A., ... Marino, M. (2005). Palmitoylation-dependent estrogen receptor α membrane localization: Regulation by 17β-estradiol. Molecular Biology of the Cell, 16(1), 231-237. https://doi.org/10.1091/mbc.E04-07-0547

Palmitoylation-dependent estrogen receptor α membrane localization : Regulation by 17β-estradiol. / Acconcia, Filippo; Ascenzi, Paolo; Bocedi, Alessio; Spisni, Enzo; Tomasi, Vittorio; Trentalance, Anna; Visca, Paolo; Marino, Maria.

In: Molecular Biology of the Cell, Vol. 16, No. 1, 01.2005, p. 231-237.

Research output: Contribution to journalArticle

Acconcia, F, Ascenzi, P, Bocedi, A, Spisni, E, Tomasi, V, Trentalance, A, Visca, P & Marino, M 2005, 'Palmitoylation-dependent estrogen receptor α membrane localization: Regulation by 17β-estradiol', Molecular Biology of the Cell, vol. 16, no. 1, pp. 231-237. https://doi.org/10.1091/mbc.E04-07-0547
Acconcia, Filippo ; Ascenzi, Paolo ; Bocedi, Alessio ; Spisni, Enzo ; Tomasi, Vittorio ; Trentalance, Anna ; Visca, Paolo ; Marino, Maria. / Palmitoylation-dependent estrogen receptor α membrane localization : Regulation by 17β-estradiol. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 1. pp. 231-237.
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