Pancreatic amylase determination by immunological and electrophoretic methods

M. Zaninotto, R. Bertorelle, S. Secchiero, M. Plebani, A. Burlina

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A new method which utilizes two monoclonal antibodies for determining pancreatic isoamylase was evaluated in comparison with the electrophoretic procedure on cellulose acetate utilized in our laboratory. Samples from 114 hospitalized patients with clinically suspected pancreatic diseases and from 70 patients affected by clinically diagnosed acute and chronic pancreatitis and pancreatic cancer, were studied. The results for pancreatic isoamylase, as determined by the monoclonal antibody method, show a not satisfactory correlation (r=0.763) with the percentage of pancreatic fraction obtained by the densitometric scanning of the electrophoretic patterns. In fact, in some pancreatic patients we encountered interpretative problems in complex isoenzymatic patterns by electrophoresis and in others a lack of agreement between measurement of pancreatic isoamylase by the two methods was observed.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalClinical Chemistry and Enzymology Communications
Issue number5
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Clinical Biochemistry


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