Pancreatic amylase determination by immunological and electrophoretic methods

M. Zaninotto, R. Bertorelle, S. Secchiero, M. Plebani, A. Burlina

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new method which utilizes two monoclonal antibodies for determining pancreatic isoamylase was evaluated in comparison with the electrophoretic procedure on cellulose acetate utilized in our laboratory. Samples from 114 hospitalized patients with clinically suspected pancreatic diseases and from 70 patients affected by clinically diagnosed acute and chronic pancreatitis and pancreatic cancer, were studied. The results for pancreatic isoamylase, as determined by the monoclonal antibody method, show a not satisfactory correlation (r=0.763) with the percentage of pancreatic fraction obtained by the densitometric scanning of the electrophoretic patterns. In fact, in some pancreatic patients we encountered interpretative problems in complex isoenzymatic patterns by electrophoresis and in others a lack of agreement between measurement of pancreatic isoamylase by the two methods was observed.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalClinical Chemistry and Enzymology Communications
Volume3
Issue number5
Publication statusPublished - 1990

Fingerprint

Isoamylase
Amylases
Monoclonal Antibodies
Electrophoresis
Pancreatic Diseases
Chronic Pancreatitis
Pancreatic Neoplasms
Scanning

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Pancreatic amylase determination by immunological and electrophoretic methods. / Zaninotto, M.; Bertorelle, R.; Secchiero, S.; Plebani, M.; Burlina, A.

In: Clinical Chemistry and Enzymology Communications, Vol. 3, No. 5, 1990, p. 261-266.

Research output: Contribution to journalArticle

Zaninotto, M. ; Bertorelle, R. ; Secchiero, S. ; Plebani, M. ; Burlina, A. / Pancreatic amylase determination by immunological and electrophoretic methods. In: Clinical Chemistry and Enzymology Communications. 1990 ; Vol. 3, No. 5. pp. 261-266.
@article{8cee1bd958b042fa9d39e84d157db62e,
title = "Pancreatic amylase determination by immunological and electrophoretic methods",
abstract = "A new method which utilizes two monoclonal antibodies for determining pancreatic isoamylase was evaluated in comparison with the electrophoretic procedure on cellulose acetate utilized in our laboratory. Samples from 114 hospitalized patients with clinically suspected pancreatic diseases and from 70 patients affected by clinically diagnosed acute and chronic pancreatitis and pancreatic cancer, were studied. The results for pancreatic isoamylase, as determined by the monoclonal antibody method, show a not satisfactory correlation (r=0.763) with the percentage of pancreatic fraction obtained by the densitometric scanning of the electrophoretic patterns. In fact, in some pancreatic patients we encountered interpretative problems in complex isoenzymatic patterns by electrophoresis and in others a lack of agreement between measurement of pancreatic isoamylase by the two methods was observed.",
author = "M. Zaninotto and R. Bertorelle and S. Secchiero and M. Plebani and A. Burlina",
year = "1990",
language = "English",
volume = "3",
pages = "261--266",
journal = "Clinical Chemistry and Enzymology Communications",
issn = "0892-2187",
publisher = "Harwood Academic Publishers",
number = "5",

}

TY - JOUR

T1 - Pancreatic amylase determination by immunological and electrophoretic methods

AU - Zaninotto, M.

AU - Bertorelle, R.

AU - Secchiero, S.

AU - Plebani, M.

AU - Burlina, A.

PY - 1990

Y1 - 1990

N2 - A new method which utilizes two monoclonal antibodies for determining pancreatic isoamylase was evaluated in comparison with the electrophoretic procedure on cellulose acetate utilized in our laboratory. Samples from 114 hospitalized patients with clinically suspected pancreatic diseases and from 70 patients affected by clinically diagnosed acute and chronic pancreatitis and pancreatic cancer, were studied. The results for pancreatic isoamylase, as determined by the monoclonal antibody method, show a not satisfactory correlation (r=0.763) with the percentage of pancreatic fraction obtained by the densitometric scanning of the electrophoretic patterns. In fact, in some pancreatic patients we encountered interpretative problems in complex isoenzymatic patterns by electrophoresis and in others a lack of agreement between measurement of pancreatic isoamylase by the two methods was observed.

AB - A new method which utilizes two monoclonal antibodies for determining pancreatic isoamylase was evaluated in comparison with the electrophoretic procedure on cellulose acetate utilized in our laboratory. Samples from 114 hospitalized patients with clinically suspected pancreatic diseases and from 70 patients affected by clinically diagnosed acute and chronic pancreatitis and pancreatic cancer, were studied. The results for pancreatic isoamylase, as determined by the monoclonal antibody method, show a not satisfactory correlation (r=0.763) with the percentage of pancreatic fraction obtained by the densitometric scanning of the electrophoretic patterns. In fact, in some pancreatic patients we encountered interpretative problems in complex isoenzymatic patterns by electrophoresis and in others a lack of agreement between measurement of pancreatic isoamylase by the two methods was observed.

UR - http://www.scopus.com/inward/record.url?scp=0025225470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025225470&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0025225470

VL - 3

SP - 261

EP - 266

JO - Clinical Chemistry and Enzymology Communications

JF - Clinical Chemistry and Enzymology Communications

SN - 0892-2187

IS - 5

ER -