Pancreatic ductal adenocarcinoma (PDA) is generally considered to have originated from pancreatic duct epithelial cells (PDEC). The ability to manipulate the growth properties of PDEC is, therefore, critical for understanding the molecular events involved in the initiation of PDA. Here, we describe methods that we have established for the isolation and maintenance of PDEC in two-dimensional and three-dimensional culture systems. The availability of these culture systems should be particularly useful for studying their relationships between specific genetic lesions and the morphogenic changes that accompany pancreatic ductal tumorigenesis.
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