Pandemic influenza A/H1N1 virus in a swine farm house in Sicily, Italy

A. Guercio, G. Purpari, P. G. Conaldi, V. Pagano, A. Moreno, P. Giambruno, L. Di Trani, G. Vaccari, E. Falcone, A. Boni, P. Cordioli

Research output: Contribution to journalArticlepeer-review


This report describes a pandemic A/H1N1 (H1N1 pdm) virus outbreak occurred in December, 2009 in a swine farm used as research facility (Istituto Mediterraneo Trapianti e Terapie ad Alta Specializzazione) for preclinical studies, located in Sicily, Italy. All the 13 pigs of the farm, showed cough, fever, inappetence and weakness. At the same time, an unvaccinated worker of the stabling showed influenza-like symptoms. RNAv extracted from two swabs collected from infected pigs resulted positive by Real Time RT-PCR for Influenza A virus. Furthermore, after growth on embryonated eggs, viral isolates were identified by Real Time RT-PCR specific for H1N1 pdm virus and characterized antigenically. Sequencing of the whole genome was also performed. All sera taken from animals and from the worker were tested by a competitive Influenza A ELISA and by the haemoagglutination inhibition test. Serological findings confirmed the circulation of influenza virus H1N1 pdm in pigs and the presence of specific antibodies against H1N1 pdm in human serum. The results of this study seem to support a H1N1 pdm transmission from man to animals showing the importance of serological and virological investigation to control the pig farms and the importance of close cooperation between the different authorities like veterinarian and human public.

Original languageEnglish
Pages (from-to)155-157
Number of pages3
JournalJournal of Environmental Biology
Issue number2
Publication statusPublished - Mar 2012


  • A/H1N1 virus
  • Pandemic influenza
  • Sequencing
  • Swine
  • Zoonosis

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Environmental Engineering
  • Toxicology


Dive into the research topics of 'Pandemic influenza A/H1N1 virus in a swine farm house in Sicily, Italy'. Together they form a unique fingerprint.

Cite this