TY - JOUR
T1 - Panton-Valentine leukocidin gene detected in a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection
AU - Montanaro, Lucio
AU - Baldassarri, Lucilla
AU - Corazzari, Tolmino
AU - Creti, Roberta
AU - Ravaioli, Stefano
AU - Cangini, Ilaria
AU - Pirini, Valter
AU - Maso, Alessandro
AU - Donati, Elena M.
AU - Pegreffi, Francesco
AU - Visai, Livia
AU - Campoccia, Davide
AU - Speziale, Pietro
AU - Arciola, Carla Renata
PY - 2009
Y1 - 2009
N2 - This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the β-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.
AB - This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the β-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.
KW - Agr polymorphisms
KW - Arthroprosthesis infection
KW - Bacterial genotyping
KW - Community acquired S. aureus (CA-MRSA and CA-MSSA)
KW - mecA gene
KW - Panton-Valentine toxin
KW - Staphylococcal adhesins
KW - Staphylococcus aureus
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M3 - Article
C2 - 19882547
AN - SCOPUS:70549096895
VL - 32
SP - 630
EP - 634
JO - International Journal of Artificial Organs
JF - International Journal of Artificial Organs
SN - 0391-3988
IS - 9
ER -