Panton-Valentine leukocidin gene detected in a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection

Lucio Montanaro, Lucilla Baldassarri, Tolmino Corazzari, Roberta Creti, Stefano Ravaioli, Ilaria Cangini, Valter Pirini, Alessandro Maso, Elena M. Donati, Francesco Pegreffi, Livia Visai, Davide Campoccia, Pietro Speziale, Carla Renata Arciola

Research output: Contribution to journalArticle

Abstract

This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the β-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.

Original languageEnglish
Pages (from-to)630-634
Number of pages5
JournalInternational Journal of Artificial Organs
Volume32
Issue number9
Publication statusPublished - 2009

Keywords

  • Agr polymorphisms
  • Arthroprosthesis infection
  • Bacterial genotyping
  • Community acquired S. aureus (CA-MRSA and CA-MSSA)
  • mecA gene
  • Panton-Valentine toxin
  • Staphylococcal adhesins
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

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  • Cite this

    Montanaro, L., Baldassarri, L., Corazzari, T., Creti, R., Ravaioli, S., Cangini, I., Pirini, V., Maso, A., Donati, E. M., Pegreffi, F., Visai, L., Campoccia, D., Speziale, P., & Arciola, C. R. (2009). Panton-Valentine leukocidin gene detected in a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection. International Journal of Artificial Organs, 32(9), 630-634.