Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption

David B. Simon, Yin Lu, Keith A. Choate, Heino Velazquez, Essam Al-Sabban, Manuel Praga, Giorgio Casari, Alberto Bettinelli, Giacomo Colussi, Juan Rodriguez-Soriano, David McCredie, David Milford, Sami Sanjad, Richard P. Lifton

Research output: Contribution to journalArticle

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Abstract

Epithelia permit selective and regulated flux from apical to basolateral surfaces by transcellular passage through cells or paracellular flux between cells. Tight junctions constitute the barrier to paracellular conductance; however, little is known about the specific molecules that mediate paracellular permeabilities. Renal magnesium ion (Mg2+) resorption occurs predominantly through a paracellular conductance in the thick ascending limb of Henle (TAL). Here, positional cloning has identified a human gene, paracellin-1 (PCLN-1), mutations on which cause renal Mg2+ wasting. PCLN-1 is located in tight junctions of the TAL and is related to the claudin family of tight junction proteins. These finding provide insight into Mg2+ homeostasis, demonstrate the role of a tight junction protein in human disease, and identify an essential component of selective paracellular conductance.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalScience
Volume285
Issue number5424
DOIs
Publication statusPublished - Jul 2 1999

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Tight Junction Proteins
Tight Junctions
Extremities
Kidney
Magnesium
Organism Cloning
Permeability
Homeostasis
Epithelium
Ions
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Simon, D. B., Lu, Y., Choate, K. A., Velazquez, H., Al-Sabban, E., Praga, M., ... Lifton, R. P. (1999). Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption. Science, 285(5424), 103-106. https://doi.org/10.1126/science.285.5424.103

Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption. / Simon, David B.; Lu, Yin; Choate, Keith A.; Velazquez, Heino; Al-Sabban, Essam; Praga, Manuel; Casari, Giorgio; Bettinelli, Alberto; Colussi, Giacomo; Rodriguez-Soriano, Juan; McCredie, David; Milford, David; Sanjad, Sami; Lifton, Richard P.

In: Science, Vol. 285, No. 5424, 02.07.1999, p. 103-106.

Research output: Contribution to journalArticle

Simon, DB, Lu, Y, Choate, KA, Velazquez, H, Al-Sabban, E, Praga, M, Casari, G, Bettinelli, A, Colussi, G, Rodriguez-Soriano, J, McCredie, D, Milford, D, Sanjad, S & Lifton, RP 1999, 'Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption', Science, vol. 285, no. 5424, pp. 103-106. https://doi.org/10.1126/science.285.5424.103
Simon DB, Lu Y, Choate KA, Velazquez H, Al-Sabban E, Praga M et al. Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption. Science. 1999 Jul 2;285(5424):103-106. https://doi.org/10.1126/science.285.5424.103
Simon, David B. ; Lu, Yin ; Choate, Keith A. ; Velazquez, Heino ; Al-Sabban, Essam ; Praga, Manuel ; Casari, Giorgio ; Bettinelli, Alberto ; Colussi, Giacomo ; Rodriguez-Soriano, Juan ; McCredie, David ; Milford, David ; Sanjad, Sami ; Lifton, Richard P. / Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption. In: Science. 1999 ; Vol. 285, No. 5424. pp. 103-106.
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