Abstract
The in vitro/in vivo growth capacity and phenotype of TS/A and the IL4- transfected TS/A-IL4 cell lines were studied by cell cycle analysis, expression of ICAM-1/CD54, transferrin receptor/CD71 and E-cadherin and by histology of the primary tumors. TS/A-IL4, unlike the TS/A line, shows in vitro a marked increase in the fibroblastoid cell type and a decreased E- cadherin expression. Administration of conditioned medium containing IL4 obtained from the TS/A-IL4 cell line, stimulates CD54 expression in the TS/A cell line. TS/A-IL4 tumors grow more slowly in vivo and are ultimately rejected. These processes are accompanied by a marked increase in collagen and extracellular matrix proteins and increased recruitment and degranulation of mast cells. The paracrine effect of IL4, released by the transfected tumor cells, might be responsible for the reduced in vivo growth of the TS/A cell line in the presence of TS/A-IL4 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 110-116 |
| Number of pages | 7 |
| Journal | Pathology and Oncology Research |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 1999 |
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Keywords
- Gene transfection
- IL-4
- TS/A adenocarcinoma
- Tumor growth
ASJC Scopus subject areas
- Cancer Research
- Oncology
- Pathology and Forensic Medicine
Cite this
Paracrine effects of IL-4 transfection on TS/A adenocarcinoma cells mediate reduced in vivo growth. / Pacor, Sabrina; Gagliardi, Renato; Spessotto, Paola; Zabucchi, Giuliano; Sava, Gianni.
In: Pathology and Oncology Research, Vol. 5, No. 2, 06.1999, p. 110-116.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Paracrine effects of IL-4 transfection on TS/A adenocarcinoma cells mediate reduced in vivo growth
AU - Pacor, Sabrina
AU - Gagliardi, Renato
AU - Spessotto, Paola
AU - Zabucchi, Giuliano
AU - Sava, Gianni
PY - 1999/6
Y1 - 1999/6
N2 - The in vitro/in vivo growth capacity and phenotype of TS/A and the IL4- transfected TS/A-IL4 cell lines were studied by cell cycle analysis, expression of ICAM-1/CD54, transferrin receptor/CD71 and E-cadherin and by histology of the primary tumors. TS/A-IL4, unlike the TS/A line, shows in vitro a marked increase in the fibroblastoid cell type and a decreased E- cadherin expression. Administration of conditioned medium containing IL4 obtained from the TS/A-IL4 cell line, stimulates CD54 expression in the TS/A cell line. TS/A-IL4 tumors grow more slowly in vivo and are ultimately rejected. These processes are accompanied by a marked increase in collagen and extracellular matrix proteins and increased recruitment and degranulation of mast cells. The paracrine effect of IL4, released by the transfected tumor cells, might be responsible for the reduced in vivo growth of the TS/A cell line in the presence of TS/A-IL4 cells.
AB - The in vitro/in vivo growth capacity and phenotype of TS/A and the IL4- transfected TS/A-IL4 cell lines were studied by cell cycle analysis, expression of ICAM-1/CD54, transferrin receptor/CD71 and E-cadherin and by histology of the primary tumors. TS/A-IL4, unlike the TS/A line, shows in vitro a marked increase in the fibroblastoid cell type and a decreased E- cadherin expression. Administration of conditioned medium containing IL4 obtained from the TS/A-IL4 cell line, stimulates CD54 expression in the TS/A cell line. TS/A-IL4 tumors grow more slowly in vivo and are ultimately rejected. These processes are accompanied by a marked increase in collagen and extracellular matrix proteins and increased recruitment and degranulation of mast cells. The paracrine effect of IL4, released by the transfected tumor cells, might be responsible for the reduced in vivo growth of the TS/A cell line in the presence of TS/A-IL4 cells.
KW - Gene transfection
KW - IL-4
KW - TS/A adenocarcinoma
KW - Tumor growth
UR - http://www.scopus.com/inward/record.url?scp=0032774049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032774049&partnerID=8YFLogxK
U2 - 10.1053/paor.1999.0172
DO - 10.1053/paor.1999.0172
M3 - Article
C2 - 10393362
AN - SCOPUS:0032774049
VL - 5
SP - 110
EP - 116
JO - Pathology and Oncology Research
JF - Pathology and Oncology Research
SN - 1219-4956
IS - 2
ER -