Paracrine effects of IL-4 transfection on TS/A adenocarcinoma cells mediate reduced in vivo growth

Sabrina Pacor, Renato Gagliardi, Paola Spessotto, Giuliano Zabucchi, Gianni Sava

Research output: Contribution to journalArticlepeer-review


The in vitro/in vivo growth capacity and phenotype of TS/A and the IL4- transfected TS/A-IL4 cell lines were studied by cell cycle analysis, expression of ICAM-1/CD54, transferrin receptor/CD71 and E-cadherin and by histology of the primary tumors. TS/A-IL4, unlike the TS/A line, shows in vitro a marked increase in the fibroblastoid cell type and a decreased E- cadherin expression. Administration of conditioned medium containing IL4 obtained from the TS/A-IL4 cell line, stimulates CD54 expression in the TS/A cell line. TS/A-IL4 tumors grow more slowly in vivo and are ultimately rejected. These processes are accompanied by a marked increase in collagen and extracellular matrix proteins and increased recruitment and degranulation of mast cells. The paracrine effect of IL4, released by the transfected tumor cells, might be responsible for the reduced in vivo growth of the TS/A cell line in the presence of TS/A-IL4 cells.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalPathology and Oncology Research
Issue number2
Publication statusPublished - Jun 1999


  • Gene transfection
  • IL-4
  • TS/A adenocarcinoma
  • Tumor growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine


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