Paracrine mechanisms of mesenchymal stem cells in tissue repair

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Tissue regeneration from transplanted mesenchymal stromal cells (MSC) either through transdifferentiation or cell fusion was originally proposed as the principal mechanism underlying their therapeutic action. However, several studies have now shown that both these mechanisms are very inefficient. The low MSC engraftment rate documented in injured areas also refutes the hypothesis that MSC repair tissue damage by replacing cell loss with newly differentiated cells. Indeed, despite evidence of preferential homing of MSC to the site of myocardial ischemia, exogenously administered MSC show poor survival and do not persist in the infarcted area. Therefore, it has been proposed that the functional benefits observed after MSC transplantation in experimental models of tissue injury might be related to the secretion of soluble factors acting in a paracrine fashion. This hypothesis is supported by pre-clinical studies demonstrating equal or even improved organ function upon infusion of MSC-derived conditioned medium (MSC-CM) compared with MSC transplantation. Identifying key MSC-secreted factors and their functional role seems a reasonable approach for a rational design of nextgeneration MSC-based therapeutics. Here, we summarize the major findings regarding both different MSC-mediated paracrine actions and the identification of paracrine mediators.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-146
Number of pages24
Volume1416
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1416
ISSN (Print)10643745

Fingerprint

Mesenchymal Stromal Cells
Cell Transplantation
Cell Fusion
Conditioned Culture Medium
Myocardial Ischemia
Regeneration
Theoretical Models

Keywords

  • Angiogenesis
  • Cell therapy
  • Conditioned medium
  • Cytoprotection
  • Mesenchymal stem cells
  • Paracrine mechanisms
  • Rigenerative medicine
  • Soluble factors
  • Tissue regeneration
  • Tissue repair

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gnecchi, M., Danieli, P., Malpasso, G., & Ciuffreda, M. C. (2016). Paracrine mechanisms of mesenchymal stem cells in tissue repair. In Methods in Molecular Biology (Vol. 1416, pp. 123-146). (Methods in Molecular Biology; Vol. 1416). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3584-0_7

Paracrine mechanisms of mesenchymal stem cells in tissue repair. / Gnecchi, Massimiliano; Danieli, Patrizia; Malpasso, Giuseppe; Ciuffreda, Maria Chiara.

Methods in Molecular Biology. Vol. 1416 Humana Press Inc., 2016. p. 123-146 (Methods in Molecular Biology; Vol. 1416).

Research output: Chapter in Book/Report/Conference proceedingChapter

Gnecchi, M, Danieli, P, Malpasso, G & Ciuffreda, MC 2016, Paracrine mechanisms of mesenchymal stem cells in tissue repair. in Methods in Molecular Biology. vol. 1416, Methods in Molecular Biology, vol. 1416, Humana Press Inc., pp. 123-146. https://doi.org/10.1007/978-1-4939-3584-0_7
Gnecchi M, Danieli P, Malpasso G, Ciuffreda MC. Paracrine mechanisms of mesenchymal stem cells in tissue repair. In Methods in Molecular Biology. Vol. 1416. Humana Press Inc. 2016. p. 123-146. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3584-0_7
Gnecchi, Massimiliano ; Danieli, Patrizia ; Malpasso, Giuseppe ; Ciuffreda, Maria Chiara. / Paracrine mechanisms of mesenchymal stem cells in tissue repair. Methods in Molecular Biology. Vol. 1416 Humana Press Inc., 2016. pp. 123-146 (Methods in Molecular Biology).
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