Parallel decrease of tissue factor surface exposure and increase of tissue factor microparticle release by the n-3 fatty acid docosahexaenoate in endothelial cells

Serena Del Turco, Giuseppina Basta, Guido Lazzerini, Monica Evangelista, Giuseppe Rainaldi, Piero Tanganelli, Marina Camera, Elena Tremoli, Raffaele De Caterina

Research output: Contribution to journalArticle

Abstract

Tissue factor (TF) is expressed on the endothelium in response to inflammatory mediators, giving endothelial cells a pro-thrombotic phenotype. Since fish-derived n-3 fatty acids (FA) have been associated with reduced incidence of myocardial infarction, we investigated the endothelial effects of the most abundant n-3 FA, docosahexaenoate (DHA), on TF expression. Human umbilical vein endothelial cells were pre-incubated with DHA (or stearate and arachidonate as controls) for 48-72 hours, and then stimulated with bacterial lipopolysaccharide (LPS) or tumor necrosis factor-α. Pre-incubation of endothelial cells with DHA (but not stearate or arachidonate) concentration-dependently reduced surface protein exposure, independent of TF mRNA or total protein expression regulation. Conversely, DHA treatment in conjunction with activating stimuli, induced the release of endothelial TF-exposing microparticles from endothelial cells, quantitatively accounting for the decreased TF cell surface exposure. In conclusion, DHA treatment, with a time-course consistent with its incorporation in membrane phospholipids, increases the release of TF-exposing microparticles from endothelial cells, accounting for decreased endothelial cell TF surface exposure, thus potentially modifying the overall endothelial control of microparticle-related effects.

Original languageEnglish
Pages (from-to)210-219
Number of pages10
JournalThrombosis and Haemostasis
Volume98
Issue number1
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Docosahexaenoic Acids
Omega-3 Fatty Acids
Thromboplastin
Endothelial Cells
Endothelium
Stearates
Human Umbilical Vein Endothelial Cells
Lipopolysaccharides
Phospholipids
Membrane Proteins
Fishes
Tumor Necrosis Factor-alpha
Myocardial Infarction
Phenotype
Messenger RNA
Membranes
Incidence

Keywords

  • Endothelial cells
  • Microparticles
  • n-3 fatty acids
  • Omega-3 fatty acids
  • Procoagulant activity
  • Tissue factor

ASJC Scopus subject areas

  • Hematology

Cite this

Parallel decrease of tissue factor surface exposure and increase of tissue factor microparticle release by the n-3 fatty acid docosahexaenoate in endothelial cells. / Del Turco, Serena; Basta, Giuseppina; Lazzerini, Guido; Evangelista, Monica; Rainaldi, Giuseppe; Tanganelli, Piero; Camera, Marina; Tremoli, Elena; De Caterina, Raffaele.

In: Thrombosis and Haemostasis, Vol. 98, No. 1, 07.2007, p. 210-219.

Research output: Contribution to journalArticle

Del Turco, Serena ; Basta, Giuseppina ; Lazzerini, Guido ; Evangelista, Monica ; Rainaldi, Giuseppe ; Tanganelli, Piero ; Camera, Marina ; Tremoli, Elena ; De Caterina, Raffaele. / Parallel decrease of tissue factor surface exposure and increase of tissue factor microparticle release by the n-3 fatty acid docosahexaenoate in endothelial cells. In: Thrombosis and Haemostasis. 2007 ; Vol. 98, No. 1. pp. 210-219.
@article{a0eb82e7254f4ccb8e60460f19fb3f57,
title = "Parallel decrease of tissue factor surface exposure and increase of tissue factor microparticle release by the n-3 fatty acid docosahexaenoate in endothelial cells",
abstract = "Tissue factor (TF) is expressed on the endothelium in response to inflammatory mediators, giving endothelial cells a pro-thrombotic phenotype. Since fish-derived n-3 fatty acids (FA) have been associated with reduced incidence of myocardial infarction, we investigated the endothelial effects of the most abundant n-3 FA, docosahexaenoate (DHA), on TF expression. Human umbilical vein endothelial cells were pre-incubated with DHA (or stearate and arachidonate as controls) for 48-72 hours, and then stimulated with bacterial lipopolysaccharide (LPS) or tumor necrosis factor-α. Pre-incubation of endothelial cells with DHA (but not stearate or arachidonate) concentration-dependently reduced surface protein exposure, independent of TF mRNA or total protein expression regulation. Conversely, DHA treatment in conjunction with activating stimuli, induced the release of endothelial TF-exposing microparticles from endothelial cells, quantitatively accounting for the decreased TF cell surface exposure. In conclusion, DHA treatment, with a time-course consistent with its incorporation in membrane phospholipids, increases the release of TF-exposing microparticles from endothelial cells, accounting for decreased endothelial cell TF surface exposure, thus potentially modifying the overall endothelial control of microparticle-related effects.",
keywords = "Endothelial cells, Microparticles, n-3 fatty acids, Omega-3 fatty acids, Procoagulant activity, Tissue factor",
author = "{Del Turco}, Serena and Giuseppina Basta and Guido Lazzerini and Monica Evangelista and Giuseppe Rainaldi and Piero Tanganelli and Marina Camera and Elena Tremoli and {De Caterina}, Raffaele",
year = "2007",
month = "7",
doi = "10.1160/TH06-07-0402",
language = "English",
volume = "98",
pages = "210--219",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "1",

}

TY - JOUR

T1 - Parallel decrease of tissue factor surface exposure and increase of tissue factor microparticle release by the n-3 fatty acid docosahexaenoate in endothelial cells

AU - Del Turco, Serena

AU - Basta, Giuseppina

AU - Lazzerini, Guido

AU - Evangelista, Monica

AU - Rainaldi, Giuseppe

AU - Tanganelli, Piero

AU - Camera, Marina

AU - Tremoli, Elena

AU - De Caterina, Raffaele

PY - 2007/7

Y1 - 2007/7

N2 - Tissue factor (TF) is expressed on the endothelium in response to inflammatory mediators, giving endothelial cells a pro-thrombotic phenotype. Since fish-derived n-3 fatty acids (FA) have been associated with reduced incidence of myocardial infarction, we investigated the endothelial effects of the most abundant n-3 FA, docosahexaenoate (DHA), on TF expression. Human umbilical vein endothelial cells were pre-incubated with DHA (or stearate and arachidonate as controls) for 48-72 hours, and then stimulated with bacterial lipopolysaccharide (LPS) or tumor necrosis factor-α. Pre-incubation of endothelial cells with DHA (but not stearate or arachidonate) concentration-dependently reduced surface protein exposure, independent of TF mRNA or total protein expression regulation. Conversely, DHA treatment in conjunction with activating stimuli, induced the release of endothelial TF-exposing microparticles from endothelial cells, quantitatively accounting for the decreased TF cell surface exposure. In conclusion, DHA treatment, with a time-course consistent with its incorporation in membrane phospholipids, increases the release of TF-exposing microparticles from endothelial cells, accounting for decreased endothelial cell TF surface exposure, thus potentially modifying the overall endothelial control of microparticle-related effects.

AB - Tissue factor (TF) is expressed on the endothelium in response to inflammatory mediators, giving endothelial cells a pro-thrombotic phenotype. Since fish-derived n-3 fatty acids (FA) have been associated with reduced incidence of myocardial infarction, we investigated the endothelial effects of the most abundant n-3 FA, docosahexaenoate (DHA), on TF expression. Human umbilical vein endothelial cells were pre-incubated with DHA (or stearate and arachidonate as controls) for 48-72 hours, and then stimulated with bacterial lipopolysaccharide (LPS) or tumor necrosis factor-α. Pre-incubation of endothelial cells with DHA (but not stearate or arachidonate) concentration-dependently reduced surface protein exposure, independent of TF mRNA or total protein expression regulation. Conversely, DHA treatment in conjunction with activating stimuli, induced the release of endothelial TF-exposing microparticles from endothelial cells, quantitatively accounting for the decreased TF cell surface exposure. In conclusion, DHA treatment, with a time-course consistent with its incorporation in membrane phospholipids, increases the release of TF-exposing microparticles from endothelial cells, accounting for decreased endothelial cell TF surface exposure, thus potentially modifying the overall endothelial control of microparticle-related effects.

KW - Endothelial cells

KW - Microparticles

KW - n-3 fatty acids

KW - Omega-3 fatty acids

KW - Procoagulant activity

KW - Tissue factor

UR - http://www.scopus.com/inward/record.url?scp=34447514130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34447514130&partnerID=8YFLogxK

U2 - 10.1160/TH06-07-0402

DO - 10.1160/TH06-07-0402

M3 - Article

C2 - 17598015

AN - SCOPUS:34447514130

VL - 98

SP - 210

EP - 219

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 1

ER -