Abstract
Purpose: Neuroendocrine differentiation is a hallmark of prostate cancer. The aim of our study was the detection of the parallel expression of neuroendocrine related markers using a prostate tissue microarray (TMA). Materials and Methods: Our study was aimed at detecting the parallel expression of NeuroD1, Chromogranin-A (ChrA), Androgen Receptor (AR) and Ki-67 by immunohistochemistry on prostate cancer tissue microarray. The data was analyzed using SAS version 8.2 (SAS Inc, Cary, NC). The relationships between NeuroD1, ChrA and AR expressions and patients' characteristics were investigated by multivariate logistic regression analysis. Progression and Overall Survival (OS) distributions were calculated using Kaplan-Meier method. Results: Tissue reactivity for NeuroD1, ChrA and AR concerned 73%, 49% and 77% of the available cases, respectively. Regarding overall survival, there were 87 deaths and 295 patients alive/censored (6 years of median follow-up). Seventy-seven disease progressions occurred at the median follow-up 5.4y. A significant correlation between NeuroD1, ChrA and AR expression was observed (p <0.001 and p <0.03, respectively). Additionally, ChrA was strongly associated in multivariate analysis to Gleason score and Ki67 expression (p <0.009 and p <0.0052, respectively). Survival analysis showed no association between markers neither for overall nor for cancer-specific survival. Conclusions: The results highlight that NeuroD1, Chromogranin-A and Androgen Receptor are strongly associated, however their expression does not correlate with overall survival or disease progression.
| Original language | English |
|---|---|
| Pages (from-to) | 57-66 |
| Number of pages | 10 |
| Journal | International Braz J Urol |
| Volume | 37 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2011 |
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Keywords
- Androgen prognosis
- Chromogranin A
- Ki-67 antigen
- NeuroD1 protein
- Neuroendocrine cells
- Prostatic neoplasms
- Receptors
ASJC Scopus subject areas
- Urology
Cite this
Parallel determination of neuroD1, chromogranin-A, KI67 and androgen receptor expression in surgically treated prostate cancers. / Cindolo, L.; Cantile, M.; Franco, R.; Chiodini, P.; Schiavo, G.; Forte, I.; Zlobec, I.; Salzano, L.; Botti, G.; Gidaro, S.; Terracciano, L.; Cillo, C.
In: International Braz J Urol, Vol. 37, No. 1, 01.2011, p. 57-66.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parallel determination of neuroD1, chromogranin-A, KI67 and androgen receptor expression in surgically treated prostate cancers
AU - Cindolo, L.
AU - Cantile, M.
AU - Franco, R.
AU - Chiodini, P.
AU - Schiavo, G.
AU - Forte, I.
AU - Zlobec, I.
AU - Salzano, L.
AU - Botti, G.
AU - Gidaro, S.
AU - Terracciano, L.
AU - Cillo, C.
PY - 2011/1
Y1 - 2011/1
N2 - Purpose: Neuroendocrine differentiation is a hallmark of prostate cancer. The aim of our study was the detection of the parallel expression of neuroendocrine related markers using a prostate tissue microarray (TMA). Materials and Methods: Our study was aimed at detecting the parallel expression of NeuroD1, Chromogranin-A (ChrA), Androgen Receptor (AR) and Ki-67 by immunohistochemistry on prostate cancer tissue microarray. The data was analyzed using SAS version 8.2 (SAS Inc, Cary, NC). The relationships between NeuroD1, ChrA and AR expressions and patients' characteristics were investigated by multivariate logistic regression analysis. Progression and Overall Survival (OS) distributions were calculated using Kaplan-Meier method. Results: Tissue reactivity for NeuroD1, ChrA and AR concerned 73%, 49% and 77% of the available cases, respectively. Regarding overall survival, there were 87 deaths and 295 patients alive/censored (6 years of median follow-up). Seventy-seven disease progressions occurred at the median follow-up 5.4y. A significant correlation between NeuroD1, ChrA and AR expression was observed (p <0.001 and p <0.03, respectively). Additionally, ChrA was strongly associated in multivariate analysis to Gleason score and Ki67 expression (p <0.009 and p <0.0052, respectively). Survival analysis showed no association between markers neither for overall nor for cancer-specific survival. Conclusions: The results highlight that NeuroD1, Chromogranin-A and Androgen Receptor are strongly associated, however their expression does not correlate with overall survival or disease progression.
AB - Purpose: Neuroendocrine differentiation is a hallmark of prostate cancer. The aim of our study was the detection of the parallel expression of neuroendocrine related markers using a prostate tissue microarray (TMA). Materials and Methods: Our study was aimed at detecting the parallel expression of NeuroD1, Chromogranin-A (ChrA), Androgen Receptor (AR) and Ki-67 by immunohistochemistry on prostate cancer tissue microarray. The data was analyzed using SAS version 8.2 (SAS Inc, Cary, NC). The relationships between NeuroD1, ChrA and AR expressions and patients' characteristics were investigated by multivariate logistic regression analysis. Progression and Overall Survival (OS) distributions were calculated using Kaplan-Meier method. Results: Tissue reactivity for NeuroD1, ChrA and AR concerned 73%, 49% and 77% of the available cases, respectively. Regarding overall survival, there were 87 deaths and 295 patients alive/censored (6 years of median follow-up). Seventy-seven disease progressions occurred at the median follow-up 5.4y. A significant correlation between NeuroD1, ChrA and AR expression was observed (p <0.001 and p <0.03, respectively). Additionally, ChrA was strongly associated in multivariate analysis to Gleason score and Ki67 expression (p <0.009 and p <0.0052, respectively). Survival analysis showed no association between markers neither for overall nor for cancer-specific survival. Conclusions: The results highlight that NeuroD1, Chromogranin-A and Androgen Receptor are strongly associated, however their expression does not correlate with overall survival or disease progression.
KW - Androgen prognosis
KW - Chromogranin A
KW - Ki-67 antigen
KW - NeuroD1 protein
KW - Neuroendocrine cells
KW - Prostatic neoplasms
KW - Receptors
UR - http://www.scopus.com/inward/record.url?scp=79955892655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955892655&partnerID=8YFLogxK
U2 - 10.1590/S1677-55382011000100008
DO - 10.1590/S1677-55382011000100008
M3 - Article
C2 - 21385481
AN - SCOPUS:79955892655
VL - 37
SP - 57
EP - 66
JO - International braz j urol : official journal of the Brazilian Society of Urology
JF - International braz j urol : official journal of the Brazilian Society of Urology
SN - 1677-5538
IS - 1
ER -