Abstract
Multifunctional nanoparticles are usually produced by sequential synthesis, with long multistep protocols. Our study reports a generic modular strategy for the parallel one-step multifunctionalization of different hydrophobic nanoparticles. The method was designed and developed by taking advantage of the natural noncovalent interactions between the fatty acid binding sites of the bovine serum albumin (BSA) and the aliphatic surfactants on different inorganic nanomaterials. As a general example of the approach, three different nanoparticles-iron oxide, upconverting nanophosphors, and gold nanospheres-were nanoemulsified in water with BSA. To support specific applications, multifunctional capability was incorporated with a variety of previously modified BSA modules. These modules include different conjugated groups, such as chelating agents for 68Ga or 89Zr and ligand molecules for enhanced in vivo targeting. A large library of 13 multimodal contrast agents was developed with this convergent strategy. This platform allows a highly versatile and easy tailoring option for efficient incorporation of functional groups. Finally, as demonstration of this versatility, a bimodal (PET/MRI) probe including a maleimide-conjugated BSA was selectively synthesized with an RGD peptide for in vivo imaging detection of tumor angiogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 153-160 |
| Number of pages | 8 |
| Journal | Bioconjugate Chemistry |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 21 2015 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Organic Chemistry
- Pharmaceutical Science
- Biomedical Engineering
- Pharmacology
- Medicine(all)
Cite this
Parallel multifunctionalization of nanoparticles : A one-step modular approach for in vivo imaging. / Groult, Hugo; Ruiz-Cabello, Jesús; Pellico, Juan; Lechuga-Vieco, Ana V.; Bhavesh, Riju; Zamai, Moreno; Almarza, Elena; Martín-Padura, Inés; Cantelar, Eugenio; Martínez-Alcázar, María P.; Herranz, Fernando.
In: Bioconjugate Chemistry, Vol. 26, No. 1, 21.01.2015, p. 153-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parallel multifunctionalization of nanoparticles
T2 - A one-step modular approach for in vivo imaging
AU - Groult, Hugo
AU - Ruiz-Cabello, Jesús
AU - Pellico, Juan
AU - Lechuga-Vieco, Ana V.
AU - Bhavesh, Riju
AU - Zamai, Moreno
AU - Almarza, Elena
AU - Martín-Padura, Inés
AU - Cantelar, Eugenio
AU - Martínez-Alcázar, María P.
AU - Herranz, Fernando
PY - 2015/1/21
Y1 - 2015/1/21
N2 - Multifunctional nanoparticles are usually produced by sequential synthesis, with long multistep protocols. Our study reports a generic modular strategy for the parallel one-step multifunctionalization of different hydrophobic nanoparticles. The method was designed and developed by taking advantage of the natural noncovalent interactions between the fatty acid binding sites of the bovine serum albumin (BSA) and the aliphatic surfactants on different inorganic nanomaterials. As a general example of the approach, three different nanoparticles-iron oxide, upconverting nanophosphors, and gold nanospheres-were nanoemulsified in water with BSA. To support specific applications, multifunctional capability was incorporated with a variety of previously modified BSA modules. These modules include different conjugated groups, such as chelating agents for 68Ga or 89Zr and ligand molecules for enhanced in vivo targeting. A large library of 13 multimodal contrast agents was developed with this convergent strategy. This platform allows a highly versatile and easy tailoring option for efficient incorporation of functional groups. Finally, as demonstration of this versatility, a bimodal (PET/MRI) probe including a maleimide-conjugated BSA was selectively synthesized with an RGD peptide for in vivo imaging detection of tumor angiogenesis.
AB - Multifunctional nanoparticles are usually produced by sequential synthesis, with long multistep protocols. Our study reports a generic modular strategy for the parallel one-step multifunctionalization of different hydrophobic nanoparticles. The method was designed and developed by taking advantage of the natural noncovalent interactions between the fatty acid binding sites of the bovine serum albumin (BSA) and the aliphatic surfactants on different inorganic nanomaterials. As a general example of the approach, three different nanoparticles-iron oxide, upconverting nanophosphors, and gold nanospheres-were nanoemulsified in water with BSA. To support specific applications, multifunctional capability was incorporated with a variety of previously modified BSA modules. These modules include different conjugated groups, such as chelating agents for 68Ga or 89Zr and ligand molecules for enhanced in vivo targeting. A large library of 13 multimodal contrast agents was developed with this convergent strategy. This platform allows a highly versatile and easy tailoring option for efficient incorporation of functional groups. Finally, as demonstration of this versatility, a bimodal (PET/MRI) probe including a maleimide-conjugated BSA was selectively synthesized with an RGD peptide for in vivo imaging detection of tumor angiogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=84921449054&partnerID=8YFLogxK
U2 - 10.1021/bc500536y
DO - 10.1021/bc500536y
M3 - Article
C2 - 25494619
AN - SCOPUS:84921449054
VL - 26
SP - 153
EP - 160
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 1
ER -