Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection

Gabriele Missale, Laura Papagno, Amalia Penna, Massimo Pilli, Alessandro Zerbini, Piero Vitali, Giovanni Pieroni, Simona Urbani, Jacopo Uggeri, Susana Pinheiro, Sarah Rowland-Jones, Carlo Ferrari

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Previous studies on CTL responses in HIV-exposed uninfected individuals assumed that the patients were exposed to replicating HIV, but the possibility that the immune responses detected were primed by exposure to a defective virus or viral antigen could not be excluded. Epidemiological and laboratory analysis of a nosocomial outbreak of acute hepatitis B unequivocally allowed the identification of an HIV-1- and HBV-co-infected patient with high plasma levels of both viruses, as the source case of the epidemics. This clinical setting provided a natural model for testing the HIV-specific T cell response in patients exposed to blood from a patient with highly replicating HIV. Parenteral exposure to both viruses led to acute hepatitis B in five subjects without evidence of HIV-1 infection. Cryopreserved lymphocytes derived from three exposed patients were tested ex vivo in an ELISPOT assay for IFN-γ release upon stimulation with peptides from structural and non-structural HIV proteins; one of the patients was also tested with four HLA/class I tetramers. Circulating HIV-specific CD8 cells were detected by tetramer staining and a high frequency of T cells were able to release IFN-γ upon stimulation with HIV peptides, showing in vivo T cell priming by HIV. These results unequivocally demonstrate a HIV-specific cell-mediated immune response in the absence of infection after exposure to highly replicating HIV.

Original languageEnglish
Pages (from-to)3208-3215
Number of pages8
JournalEuropean Journal of Immunology
Volume34
Issue number11
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Viremia
HIV
Viruses
T-Lymphocytes
Infection
Hepatitis B
HIV-1
Human Immunodeficiency Virus Proteins
Defective Viruses
Enzyme-Linked Immunospot Assay
Peptides
Viral Antigens
HIV Infections
Disease Outbreaks
Lymphocytes
Staining and Labeling

Keywords

  • HBV
  • HIV
  • T cell response

ASJC Scopus subject areas

  • Immunology

Cite this

Missale, G., Papagno, L., Penna, A., Pilli, M., Zerbini, A., Vitali, P., ... Ferrari, C. (2004). Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. European Journal of Immunology, 34(11), 3208-3215. https://doi.org/10.1002/eji.200424889

Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. / Missale, Gabriele; Papagno, Laura; Penna, Amalia; Pilli, Massimo; Zerbini, Alessandro; Vitali, Piero; Pieroni, Giovanni; Urbani, Simona; Uggeri, Jacopo; Pinheiro, Susana; Rowland-Jones, Sarah; Ferrari, Carlo.

In: European Journal of Immunology, Vol. 34, No. 11, 11.2004, p. 3208-3215.

Research output: Contribution to journalArticle

Missale, G, Papagno, L, Penna, A, Pilli, M, Zerbini, A, Vitali, P, Pieroni, G, Urbani, S, Uggeri, J, Pinheiro, S, Rowland-Jones, S & Ferrari, C 2004, 'Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection', European Journal of Immunology, vol. 34, no. 11, pp. 3208-3215. https://doi.org/10.1002/eji.200424889
Missale, Gabriele ; Papagno, Laura ; Penna, Amalia ; Pilli, Massimo ; Zerbini, Alessandro ; Vitali, Piero ; Pieroni, Giovanni ; Urbani, Simona ; Uggeri, Jacopo ; Pinheiro, Susana ; Rowland-Jones, Sarah ; Ferrari, Carlo. / Parenteral exposure to high HIV viremia leads to virus-specific T cell priming without evidence of infection. In: European Journal of Immunology. 2004 ; Vol. 34, No. 11. pp. 3208-3215.
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