PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage

Sara Martire, Andrea Fuso, Dante Rotili, Italo Tempera, Cesare Giordano, Ivana De Zottis, Alessia Muzi, Patrizia Vernole, Grazia Graziani, Emanuela Lococo, Martina Faraldi, Bruno Maras, Sigfrido Scarpa, Luciana Mosca, Maria d'Erme

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Amyloid beta peptide (Aβ) causes neurodegeneration by several mechanisms including oxidative stress, which is known to induce DNA damage with the consequent activation of poly (ADP-ribose) polymerase (PARP-1). To elucidate the role of PARP-1 in the neurodegenerative process, SH-SY5Y neuroblastoma cells were treated with Aβ25-35 fragment in the presence or absence of MC2050, a new PARP-1 inhibitor. Aβ25-35 induces an enhancement of PARP activity which is prevented by cell pre-treatment with MC2050. These data were confirmed by measuring PARP-1 activity in CHO cells transfected with amylod precursor protein and in vivo in brains specimens of TgCRND8 transgenic mice overproducing the amyloid peptide. Following Aβ25-35 exposure a significant increase in intracellular ROS was observed. These data were supported by the finding that Aβ25-35 induces DNA damage which in turn activates PARP-1. Challenge with Aβ25-35 is also able to activate NF-kB via PARP-1, as demonstrated by NF-kB impairment upon MC2050 treatment. Moreover, Aβ25-35 via PARP-1 induces a significant increase in the p53 protein level and a parallel decrease in the anti-apoptotic Bcl-2 protein. These overall data support the hypothesis of PARP-1 involvment in cellular responses induced by Aβ and hence a possible rationale for the implication of PARP-1 in neurodegeneration is discussed.

Original languageEnglish
Article numbere72169
JournalPLoS One
Volume8
Issue number9
DOIs
Publication statusPublished - Sep 24 2013

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NF-kappa B
Amyloid beta-Peptides
amyloid
DNA Damage
transcription factor NF-kappa B
peptides
DNA damage
Oxidative stress
Protein Precursors
Poly(ADP-ribose) Polymerases
CHO Cells
DNA
Neuroblastoma
Amyloid
Transgenic Mice
NAD ADP-ribosyltransferase
Brain
Proteins
Oxidative Stress
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Martire, S., Fuso, A., Rotili, D., Tempera, I., Giordano, C., De Zottis, I., ... d'Erme, M. (2013). PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage. PLoS One, 8(9), [e72169]. https://doi.org/10.1371/journal.pone.0072169

PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage. / Martire, Sara; Fuso, Andrea; Rotili, Dante; Tempera, Italo; Giordano, Cesare; De Zottis, Ivana; Muzi, Alessia; Vernole, Patrizia; Graziani, Grazia; Lococo, Emanuela; Faraldi, Martina; Maras, Bruno; Scarpa, Sigfrido; Mosca, Luciana; d'Erme, Maria.

In: PLoS One, Vol. 8, No. 9, e72169, 24.09.2013.

Research output: Contribution to journalArticle

Martire, S, Fuso, A, Rotili, D, Tempera, I, Giordano, C, De Zottis, I, Muzi, A, Vernole, P, Graziani, G, Lococo, E, Faraldi, M, Maras, B, Scarpa, S, Mosca, L & d'Erme, M 2013, 'PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage', PLoS One, vol. 8, no. 9, e72169. https://doi.org/10.1371/journal.pone.0072169
Martire S, Fuso A, Rotili D, Tempera I, Giordano C, De Zottis I et al. PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage. PLoS One. 2013 Sep 24;8(9). e72169. https://doi.org/10.1371/journal.pone.0072169
Martire, Sara ; Fuso, Andrea ; Rotili, Dante ; Tempera, Italo ; Giordano, Cesare ; De Zottis, Ivana ; Muzi, Alessia ; Vernole, Patrizia ; Graziani, Grazia ; Lococo, Emanuela ; Faraldi, Martina ; Maras, Bruno ; Scarpa, Sigfrido ; Mosca, Luciana ; d'Erme, Maria. / PARP-1 Modulates Amyloid Beta Peptide-Induced Neuronal Damage. In: PLoS One. 2013 ; Vol. 8, No. 9.
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