Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa

Claudio Sette, Arturo Bevilacqua, Andrea Bianchini, Franco Mangia, Raffaele Geremia, Pellegrino Rossi

Research output: Contribution to journalArticle

Abstract

A truncated form of the c-kit tyrosine kinase receptor, corresponding to the phosphotransferase portion of the cytoplasmic catalytic domain and the carboxyterminus (tr-kit), is accumulated during late mouse spermiogenesis. Here we report that tr-kit is specifically localized in the residual sperm cytoplasm, with maximal accumulation in the midpiece of the flagellum, suggesting that it can enter the egg during fertilization. Microinjection of extracts from COS cells expressing a recombinant tr-kit protein into metaphase II-arrested mouse oocytes caused complete oocyte activation, including cortical granule exocytosis, completion of the 2nd meiotic division, formation of a parthenogenetic pronucleus and progression through cleavage stages. No activation above background levels was obtained with extracts from mock-transfected COS cells. Similar results were obtained by microinjection of in vitro synthesized tr-kit mRNA into metaphase II-arrested oocytes. Tr-kit-induced parthenogenetic egg activation was completely inhibited by oocyte preincubation with the Ca2+-chelating agent BAPTA-AM or with a specific inhibitor of phospholipase C activity. Tr-kit-induced egg activation was associated with a decrease in activity of mitogen-activated protein kinase, an essential component of the cytostatic factor. These results candidate tr-kit as a putative sperm factor required for triggering activation of mouse eggs at fertilization.

Original languageEnglish
Pages (from-to)2267-2274
Number of pages8
JournalDevelopment
Volume124
Issue number11
Publication statusPublished - Jun 1997

Fingerprint

Microinjections
Protein-Tyrosine Kinases
Eggs
Oocytes
Spermatozoa
Ovum
COS Cells
Metaphase
Fertilization
Proto-Oncogene Proteins c-mos
Flagella
Exocytosis
Receptor Protein-Tyrosine Kinases
Type C Phospholipases
Spermatogenesis
Chelating Agents
Mitogen-Activated Protein Kinases
Catalytic Domain
Cytoplasm
Phosphotransferases

Keywords

  • c-kit
  • Calcium
  • Egg activation
  • MAP kinase
  • Metaphase II-arrest
  • Phospholipase C
  • Spermatozoa
  • Truncated-receptor
  • Tyrosine-kinase

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Sette, C., Bevilacqua, A., Bianchini, A., Mangia, F., Geremia, R., & Rossi, P. (1997). Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa. Development, 124(11), 2267-2274.

Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa. / Sette, Claudio; Bevilacqua, Arturo; Bianchini, Andrea; Mangia, Franco; Geremia, Raffaele; Rossi, Pellegrino.

In: Development, Vol. 124, No. 11, 06.1997, p. 2267-2274.

Research output: Contribution to journalArticle

Sette, C, Bevilacqua, A, Bianchini, A, Mangia, F, Geremia, R & Rossi, P 1997, 'Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa', Development, vol. 124, no. 11, pp. 2267-2274.
Sette, Claudio ; Bevilacqua, Arturo ; Bianchini, Andrea ; Mangia, Franco ; Geremia, Raffaele ; Rossi, Pellegrino. / Parthenogenetic activation of mouse eggs by microinjection of a truncated c-kit tyrosine kinase present in spermatozoa. In: Development. 1997 ; Vol. 124, No. 11. pp. 2267-2274.
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