Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats

M. A. Macrì, R. Campanella, G. Garreffa, M. Occhigrossi, F. De Luca, E. Arrigoni Martelli, B. Maraviglia

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31P NMR spectroscopy was used to assess the cerebral ischemia status in rats by measuring the relative levels of phosphate metabolites. Partial cerebral ischemia was induced in 49 rats by reversible occlusion of the carotid arteries. Rats were intubated and mechanically ventilated on a hypoxic gas mixture. Physiological parameters such as temperature and arterial pressure were strictly controlled during the experiments. 31P spectra were acquired at 7 T during basal observation, for 15-20 min after the induction of ischemia, and for 1 hr after reperfusion. Depletion and increase in PCr and Pi levels, respectively, were already observable in the collected spectra within few minutes after the onset of ischemia. No appreciable changes were found in the ATP levels.

Original languageEnglish
Pages (from-to)769-772
Number of pages4
JournalMagnetic Resonance Imaging
Issue number5
Publication statusPublished - 1992


  • P NMR spectroscopy
  • Cerebral ischemia
  • Hypoxia
  • Reperfusion

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics


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