Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats

M. A. Macrì, R. Campanella, G. Garreffa, M. Occhigrossi, F. De Luca, E. Arrigoni Martelli, B. Maraviglia

Research output: Contribution to journalArticle

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Abstract

31P NMR spectroscopy was used to assess the cerebral ischemia status in rats by measuring the relative levels of phosphate metabolites. Partial cerebral ischemia was induced in 49 rats by reversible occlusion of the carotid arteries. Rats were intubated and mechanically ventilated on a hypoxic gas mixture. Physiological parameters such as temperature and arterial pressure were strictly controlled during the experiments. 31P spectra were acquired at 7 T during basal observation, for 15-20 min after the induction of ischemia, and for 1 hr after reperfusion. Depletion and increase in PCr and Pi levels, respectively, were already observable in the collected spectra within few minutes after the onset of ischemia. No appreciable changes were found in the ATP levels.

Original languageEnglish
Pages (from-to)769-772
Number of pages4
JournalMagnetic Resonance Imaging
Volume10
Issue number5
DOIs
Publication statusPublished - 1992

Fingerprint

ischemia
Brain Ischemia
Nuclear magnetic resonance spectroscopy
rats
Rats
Magnetic Resonance Spectroscopy
nuclear magnetic resonance
Ischemia
spectroscopy
Adenosinetriphosphate
Metabolites
Carotid Arteries
Gas mixtures
Reperfusion
adenosine triphosphate
metabolites
occlusion
Arterial Pressure
Phosphates
arteries

Keywords

  • P NMR spectroscopy
  • Cerebral ischemia
  • Hypoxia
  • Reperfusion

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Macrì, M. A., Campanella, R., Garreffa, G., Occhigrossi, M., De Luca, F., Arrigoni Martelli, E., & Maraviglia, B. (1992). Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats. Magnetic Resonance Imaging, 10(5), 769-772. https://doi.org/10.1016/0730-725X(92)90410-2

Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats. / Macrì, M. A.; Campanella, R.; Garreffa, G.; Occhigrossi, M.; De Luca, F.; Arrigoni Martelli, E.; Maraviglia, B.

In: Magnetic Resonance Imaging, Vol. 10, No. 5, 1992, p. 769-772.

Research output: Contribution to journalArticle

Macrì, MA, Campanella, R, Garreffa, G, Occhigrossi, M, De Luca, F, Arrigoni Martelli, E & Maraviglia, B 1992, 'Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats', Magnetic Resonance Imaging, vol. 10, no. 5, pp. 769-772. https://doi.org/10.1016/0730-725X(92)90410-2
Macrì MA, Campanella R, Garreffa G, Occhigrossi M, De Luca F, Arrigoni Martelli E et al. Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats. Magnetic Resonance Imaging. 1992;10(5):769-772. https://doi.org/10.1016/0730-725X(92)90410-2
Macrì, M. A. ; Campanella, R. ; Garreffa, G. ; Occhigrossi, M. ; De Luca, F. ; Arrigoni Martelli, E. ; Maraviglia, B. / Partial cerebral ischemia assessed by "in vivo" 31P NMR spectroscopy in rats. In: Magnetic Resonance Imaging. 1992 ; Vol. 10, No. 5. pp. 769-772.
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