Partial characterisation of a minichromosome derived from human chromosome 13

G. Malferrari, B. Castiglioni, M. Rocchi, V. Sgaramella, I. Biunno

Research output: Contribution to journalArticlepeer-review

Abstract

This paper reports the partial characterisation of a newly generated minichromosome contained within the hybrid cell line RH13L6. We show that the minichromosome contains: chromosome 13 alphoid repeats, Alu sequences and part of the q22 region of human chromosome 13. We tested the presence of several markers positioned in the pericentromeric and subtelomeric regions. We found that the minichromosome is mitotically unstable in the host cells and after few doubling is no longer detectable. Although minichromosomes or artificial chromosomes are considered promising cloning and expression vectors, especially for therapeutic purposes, this minichromosome could only be used in conditions where transient expression of transgene(s) is required.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalTransgenics
Volume3
Issue number2-4
Publication statusPublished - 2001

Keywords

  • Alphoids
  • Alu sequences
  • Centromere
  • FISH
  • Human artificial chromosomes

ASJC Scopus subject areas

  • Genetics

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