Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens

Susanna Esposito, Alessia Scala, Sonia Bianchini, Maria Lory Presicce, Alessandro Mori, Calogero Sathya Sciarrabba, Giulia Fior, Nicola Principi

Research output: Contribution to journalArticle

Abstract

In this study, 185 nasopharyngeal swabs were tested to compare the sensitivity and specificity of the Luminex NxTAG (NxTAG) Respiratory Pathogen Panel (RPP) Assay with those of the Luminex Respiratory Virus Panel (RVP) Fast Assay v2 and singleplex real-time polymerase chain reaction (PCR). The NxTAG Assay identified at least one infectious agent in 164 (88.7%) of the swabs. In 91 (6.2%) tests with negative results with the RVP Fast Assay v2, a virus was identified by the NxTAG (P < 0.001). With the NxTAG Assay, the detection rates were significantly higher for respiratory syncytial virus (P = 0.003), human metapneumovirus (P < 0.001), human rhinovirus/human enterovirus (P = 0.009) and human adenovirus (P < 0.001). Finally, the NxTAG Assay identified M. pneumoniae in 32 of 44 (72.7%) PCR-positive samples. However, the concordance with real-time PCR results was low for both assays. In conclusion, the results indicate that the NxTAG Assay overcomes some of the limitations of previous Luminex assays, although further studies are needed for a more complete evaluation of the new assay.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume86
Issue number1
DOIs
Publication statusPublished - Sep 1 2016

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Real-Time Polymerase Chain Reaction
Viruses
Metapneumovirus
Human Adenoviruses
Rhinovirus
Respiratory Syncytial Viruses
Enterovirus
Pneumonia
Sensitivity and Specificity
Polymerase Chain Reaction

Keywords

  • Atypical bacteria
  • Luminex NxTAG Respiratory Pathogen Panel Assay
  • Luminex Respiratory Virus Panel Fast Assay v2
  • Real-time polymerase chain reaction
  • Respiratory viruses

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens. / Esposito, Susanna; Scala, Alessia; Bianchini, Sonia; Presicce, Maria Lory; Mori, Alessandro; Sciarrabba, Calogero Sathya; Fior, Giulia; Principi, Nicola.

In: Diagnostic Microbiology and Infectious Disease, Vol. 86, No. 1, 01.09.2016, p. 53-57.

Research output: Contribution to journalArticle

Esposito, Susanna ; Scala, Alessia ; Bianchini, Sonia ; Presicce, Maria Lory ; Mori, Alessandro ; Sciarrabba, Calogero Sathya ; Fior, Giulia ; Principi, Nicola. / Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens. In: Diagnostic Microbiology and Infectious Disease. 2016 ; Vol. 86, No. 1. pp. 53-57.
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