Particulate air pollution exposure and expression of viral and human MicroRNAs in blood

The beijing truck driver air pollution study

Lifang Hou, Jitendra Barupal, Wei Zhang, Yinan Zheng, Lei Liu, Xiao Zhang, Chang Dou, John P. McCracken, Anaité Díaz, Valeria Motta, Marco Sanchez-Guerra, Katherine Rose Wolf, Pier Alberto Bertazzi, Joel D. Schwartz, Sheng Wang, Andrea A. Baccarelli

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: MicroRNAs (miRNAs) are post-transcriptional gene suppressors and potential mediators of environmental effects. In addition to human miRNAs, viral miRNAs expressed from latent viral sequences are detectable in human cells. Objective: In a highly exposed population in Beijing, China, we evaluated the associations of particulate air pollution exposure on blood miRNA profiles. Methods: The Beijing Truck Driver Air Pollution Study (BTDAS) included 60 truck drivers and 60 office workers. We investigated associations of short-term air pollution exposure, using measures of personal PM2.5 (particulate matter ≤ 2.5 μm) and elemental carbon (EC), and ambient PM10 (≤ 10 μm), with blood NanoString nCounter miRNA profiles at two exams separated by 1–2 weeks. Results: No miRNA was significantly associated with personal PM2.5 at a false discovery rate (FDR) of 20%. Short-term ambient PM10 was associated with the expression of 12 miRNAs in office workers only (FDR <20%). Short-term EC was associated with differential expression of 46 human and 7 viral miRNAs, the latter including 3 and 4 viral miRNAs in office workers and truck drivers, respectively. EC-associated miRNAs differed between office workers and truck drivers with significant effect modification by occupational group. Functional interaction network analysis suggested enriched cellular proliferation/differentiation pathways in truck drivers and proinflammation pathways in office workers. Conclusions: Short-term EC exposure was associated with the expression of human and viral miRNAs that may influence immune responses and other biological pathways. Associations between EC exposure and viral miRNA expression suggest that latent viral miRNAs are potential mediators of air pollution–associated health effects. PM2.5/PM10 exposures showed no consistent relationships with miRNA expression.

Original languageEnglish
Pages (from-to)344-350
Number of pages7
JournalEnvironmental Health Perspectives
Volume124
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Air Pollution
Motor Vehicles
MicroRNAs
Carbon
Particulate Matter
Beijing
Suppressor Genes
Occupational Groups
China
Air
Cell Proliferation

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Particulate air pollution exposure and expression of viral and human MicroRNAs in blood : The beijing truck driver air pollution study. / Hou, Lifang; Barupal, Jitendra; Zhang, Wei; Zheng, Yinan; Liu, Lei; Zhang, Xiao; Dou, Chang; McCracken, John P.; Díaz, Anaité; Motta, Valeria; Sanchez-Guerra, Marco; Wolf, Katherine Rose; Bertazzi, Pier Alberto; Schwartz, Joel D.; Wang, Sheng; Baccarelli, Andrea A.

In: Environmental Health Perspectives, Vol. 124, No. 3, 01.03.2016, p. 344-350.

Research output: Contribution to journalArticle

Hou, L, Barupal, J, Zhang, W, Zheng, Y, Liu, L, Zhang, X, Dou, C, McCracken, JP, Díaz, A, Motta, V, Sanchez-Guerra, M, Wolf, KR, Bertazzi, PA, Schwartz, JD, Wang, S & Baccarelli, AA 2016, 'Particulate air pollution exposure and expression of viral and human MicroRNAs in blood: The beijing truck driver air pollution study', Environmental Health Perspectives, vol. 124, no. 3, pp. 344-350. https://doi.org/10.1289/ehp.1408519
Hou, Lifang ; Barupal, Jitendra ; Zhang, Wei ; Zheng, Yinan ; Liu, Lei ; Zhang, Xiao ; Dou, Chang ; McCracken, John P. ; Díaz, Anaité ; Motta, Valeria ; Sanchez-Guerra, Marco ; Wolf, Katherine Rose ; Bertazzi, Pier Alberto ; Schwartz, Joel D. ; Wang, Sheng ; Baccarelli, Andrea A. / Particulate air pollution exposure and expression of viral and human MicroRNAs in blood : The beijing truck driver air pollution study. In: Environmental Health Perspectives. 2016 ; Vol. 124, No. 3. pp. 344-350.
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T2 - The beijing truck driver air pollution study

AU - Hou, Lifang

AU - Barupal, Jitendra

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AU - Zheng, Yinan

AU - Liu, Lei

AU - Zhang, Xiao

AU - Dou, Chang

AU - McCracken, John P.

AU - Díaz, Anaité

AU - Motta, Valeria

AU - Sanchez-Guerra, Marco

AU - Wolf, Katherine Rose

AU - Bertazzi, Pier Alberto

AU - Schwartz, Joel D.

AU - Wang, Sheng

AU - Baccarelli, Andrea A.

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N2 - Background: MicroRNAs (miRNAs) are post-transcriptional gene suppressors and potential mediators of environmental effects. In addition to human miRNAs, viral miRNAs expressed from latent viral sequences are detectable in human cells. Objective: In a highly exposed population in Beijing, China, we evaluated the associations of particulate air pollution exposure on blood miRNA profiles. Methods: The Beijing Truck Driver Air Pollution Study (BTDAS) included 60 truck drivers and 60 office workers. We investigated associations of short-term air pollution exposure, using measures of personal PM2.5 (particulate matter ≤ 2.5 μm) and elemental carbon (EC), and ambient PM10 (≤ 10 μm), with blood NanoString nCounter miRNA profiles at two exams separated by 1–2 weeks. Results: No miRNA was significantly associated with personal PM2.5 at a false discovery rate (FDR) of 20%. Short-term ambient PM10 was associated with the expression of 12 miRNAs in office workers only (FDR <20%). Short-term EC was associated with differential expression of 46 human and 7 viral miRNAs, the latter including 3 and 4 viral miRNAs in office workers and truck drivers, respectively. EC-associated miRNAs differed between office workers and truck drivers with significant effect modification by occupational group. Functional interaction network analysis suggested enriched cellular proliferation/differentiation pathways in truck drivers and proinflammation pathways in office workers. Conclusions: Short-term EC exposure was associated with the expression of human and viral miRNAs that may influence immune responses and other biological pathways. Associations between EC exposure and viral miRNA expression suggest that latent viral miRNAs are potential mediators of air pollution–associated health effects. PM2.5/PM10 exposures showed no consistent relationships with miRNA expression.

AB - Background: MicroRNAs (miRNAs) are post-transcriptional gene suppressors and potential mediators of environmental effects. In addition to human miRNAs, viral miRNAs expressed from latent viral sequences are detectable in human cells. Objective: In a highly exposed population in Beijing, China, we evaluated the associations of particulate air pollution exposure on blood miRNA profiles. Methods: The Beijing Truck Driver Air Pollution Study (BTDAS) included 60 truck drivers and 60 office workers. We investigated associations of short-term air pollution exposure, using measures of personal PM2.5 (particulate matter ≤ 2.5 μm) and elemental carbon (EC), and ambient PM10 (≤ 10 μm), with blood NanoString nCounter miRNA profiles at two exams separated by 1–2 weeks. Results: No miRNA was significantly associated with personal PM2.5 at a false discovery rate (FDR) of 20%. Short-term ambient PM10 was associated with the expression of 12 miRNAs in office workers only (FDR <20%). Short-term EC was associated with differential expression of 46 human and 7 viral miRNAs, the latter including 3 and 4 viral miRNAs in office workers and truck drivers, respectively. EC-associated miRNAs differed between office workers and truck drivers with significant effect modification by occupational group. Functional interaction network analysis suggested enriched cellular proliferation/differentiation pathways in truck drivers and proinflammation pathways in office workers. Conclusions: Short-term EC exposure was associated with the expression of human and viral miRNAs that may influence immune responses and other biological pathways. Associations between EC exposure and viral miRNA expression suggest that latent viral miRNAs are potential mediators of air pollution–associated health effects. PM2.5/PM10 exposures showed no consistent relationships with miRNA expression.

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