Passive Smoking Exacerbates Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Isoform 2-Induced Oxidative Stress and Arterial Dysfunction in Children with Persistent Allergic Rhinitis

Lorenzo Loffredo, Anna Maria Zicari, Francesca Occasi, Ludovica Perri, Roberto Carnevale, Simona Battaglia, Francesco Angelico, Maria Del Ben, Francesco Martino, Cristina Nocella, Alessio Farcomeni, Giovanna De Castro, Marzia Duse, Francesco Violi

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To characterize nicotinamide-adenine dinucleotide phosphate oxidase isoform 2 (NOX2), oxidative stress, and endothelial function in children with and without allergic rhinitis and to ascertain the effect of passive smoke exposure on these factors, because there is an established association between allergic rhinitis and increased cardiovascular risk in adults.

METHODS: We recruited 130 children-65 with persistent allergic rhinitis and 65 healthy controls. A cross-sectional study was performed to compare endothelial function by flow-mediated dilation, blood levels of isoprostanes, serum activity of soluble NOX2-dp (sNOX2-dp), and nitric oxide bioavailability, in these 2 groups of children. Serum cotinine levels were assessed to measure exposure to passive smoking.

RESULTS: Compared with healthy controls, children with persistent allergic rhinitis had significantly higher sNOX2-dp and isoprostanes levels, lower flow-mediated dilation, and reduced nitric oxide bioavailability. Multivariable linear regression analysis showed that flow-mediated dilation, isoprostanes, and cotinine were independently associated with sNOX2-dp levels. Of note, sNOX2-dp serum levels were significantly higher in children with allergic rhinitis exposed to smoke, as compared with unexposed children with allergic rhinitis.

CONCLUSION: NOX2 is activated in children with persistent allergic rhinitis and passive smoke exposure exacerbates this effect. We further demonstrate an association between higher sNOX2-dp and oxidative stress and endothelial dysfunction.

Original languageEnglish
Pages (from-to)252-257
Number of pages6
JournalJournal of Pediatrics
Volume202
DOIs
Publication statusPublished - Nov 2018

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Tobacco Smoke Pollution
NADP
Oxidoreductases
Protein Isoforms
Oxidative Stress
Isoprostanes
Smoke
Dilatation
Cotinine
Biological Availability
Nitric Oxide
Serum
Allergic Rhinitis
Linear Models
Cross-Sectional Studies
Regression Analysis

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Passive Smoking Exacerbates Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Isoform 2-Induced Oxidative Stress and Arterial Dysfunction in Children with Persistent Allergic Rhinitis. / Loffredo, Lorenzo; Zicari, Anna Maria; Occasi, Francesca; Perri, Ludovica; Carnevale, Roberto; Battaglia, Simona; Angelico, Francesco; Del Ben, Maria; Martino, Francesco; Nocella, Cristina; Farcomeni, Alessio; De Castro, Giovanna; Duse, Marzia; Violi, Francesco.

In: Journal of Pediatrics, Vol. 202, 11.2018, p. 252-257.

Research output: Contribution to journalArticle

Loffredo, L, Zicari, AM, Occasi, F, Perri, L, Carnevale, R, Battaglia, S, Angelico, F, Del Ben, M, Martino, F, Nocella, C, Farcomeni, A, De Castro, G, Duse, M & Violi, F 2018, 'Passive Smoking Exacerbates Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Isoform 2-Induced Oxidative Stress and Arterial Dysfunction in Children with Persistent Allergic Rhinitis', Journal of Pediatrics, vol. 202, pp. 252-257. https://doi.org/10.1016/j.jpeds.2018.06.053
Loffredo, Lorenzo ; Zicari, Anna Maria ; Occasi, Francesca ; Perri, Ludovica ; Carnevale, Roberto ; Battaglia, Simona ; Angelico, Francesco ; Del Ben, Maria ; Martino, Francesco ; Nocella, Cristina ; Farcomeni, Alessio ; De Castro, Giovanna ; Duse, Marzia ; Violi, Francesco. / Passive Smoking Exacerbates Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Isoform 2-Induced Oxidative Stress and Arterial Dysfunction in Children with Persistent Allergic Rhinitis. In: Journal of Pediatrics. 2018 ; Vol. 202. pp. 252-257.
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abstract = "OBJECTIVE: To characterize nicotinamide-adenine dinucleotide phosphate oxidase isoform 2 (NOX2), oxidative stress, and endothelial function in children with and without allergic rhinitis and to ascertain the effect of passive smoke exposure on these factors, because there is an established association between allergic rhinitis and increased cardiovascular risk in adults.METHODS: We recruited 130 children-65 with persistent allergic rhinitis and 65 healthy controls. A cross-sectional study was performed to compare endothelial function by flow-mediated dilation, blood levels of isoprostanes, serum activity of soluble NOX2-dp (sNOX2-dp), and nitric oxide bioavailability, in these 2 groups of children. Serum cotinine levels were assessed to measure exposure to passive smoking.RESULTS: Compared with healthy controls, children with persistent allergic rhinitis had significantly higher sNOX2-dp and isoprostanes levels, lower flow-mediated dilation, and reduced nitric oxide bioavailability. Multivariable linear regression analysis showed that flow-mediated dilation, isoprostanes, and cotinine were independently associated with sNOX2-dp levels. Of note, sNOX2-dp serum levels were significantly higher in children with allergic rhinitis exposed to smoke, as compared with unexposed children with allergic rhinitis.CONCLUSION: NOX2 is activated in children with persistent allergic rhinitis and passive smoke exposure exacerbates this effect. We further demonstrate an association between higher sNOX2-dp and oxidative stress and endothelial dysfunction.",
author = "Lorenzo Loffredo and Zicari, {Anna Maria} and Francesca Occasi and Ludovica Perri and Roberto Carnevale and Simona Battaglia and Francesco Angelico and {Del Ben}, Maria and Francesco Martino and Cristina Nocella and Alessio Farcomeni and {De Castro}, Giovanna and Marzia Duse and Francesco Violi",
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T1 - Passive Smoking Exacerbates Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Isoform 2-Induced Oxidative Stress and Arterial Dysfunction in Children with Persistent Allergic Rhinitis

AU - Loffredo, Lorenzo

AU - Zicari, Anna Maria

AU - Occasi, Francesca

AU - Perri, Ludovica

AU - Carnevale, Roberto

AU - Battaglia, Simona

AU - Angelico, Francesco

AU - Del Ben, Maria

AU - Martino, Francesco

AU - Nocella, Cristina

AU - Farcomeni, Alessio

AU - De Castro, Giovanna

AU - Duse, Marzia

AU - Violi, Francesco

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018/11

Y1 - 2018/11

N2 - OBJECTIVE: To characterize nicotinamide-adenine dinucleotide phosphate oxidase isoform 2 (NOX2), oxidative stress, and endothelial function in children with and without allergic rhinitis and to ascertain the effect of passive smoke exposure on these factors, because there is an established association between allergic rhinitis and increased cardiovascular risk in adults.METHODS: We recruited 130 children-65 with persistent allergic rhinitis and 65 healthy controls. A cross-sectional study was performed to compare endothelial function by flow-mediated dilation, blood levels of isoprostanes, serum activity of soluble NOX2-dp (sNOX2-dp), and nitric oxide bioavailability, in these 2 groups of children. Serum cotinine levels were assessed to measure exposure to passive smoking.RESULTS: Compared with healthy controls, children with persistent allergic rhinitis had significantly higher sNOX2-dp and isoprostanes levels, lower flow-mediated dilation, and reduced nitric oxide bioavailability. Multivariable linear regression analysis showed that flow-mediated dilation, isoprostanes, and cotinine were independently associated with sNOX2-dp levels. Of note, sNOX2-dp serum levels were significantly higher in children with allergic rhinitis exposed to smoke, as compared with unexposed children with allergic rhinitis.CONCLUSION: NOX2 is activated in children with persistent allergic rhinitis and passive smoke exposure exacerbates this effect. We further demonstrate an association between higher sNOX2-dp and oxidative stress and endothelial dysfunction.

AB - OBJECTIVE: To characterize nicotinamide-adenine dinucleotide phosphate oxidase isoform 2 (NOX2), oxidative stress, and endothelial function in children with and without allergic rhinitis and to ascertain the effect of passive smoke exposure on these factors, because there is an established association between allergic rhinitis and increased cardiovascular risk in adults.METHODS: We recruited 130 children-65 with persistent allergic rhinitis and 65 healthy controls. A cross-sectional study was performed to compare endothelial function by flow-mediated dilation, blood levels of isoprostanes, serum activity of soluble NOX2-dp (sNOX2-dp), and nitric oxide bioavailability, in these 2 groups of children. Serum cotinine levels were assessed to measure exposure to passive smoking.RESULTS: Compared with healthy controls, children with persistent allergic rhinitis had significantly higher sNOX2-dp and isoprostanes levels, lower flow-mediated dilation, and reduced nitric oxide bioavailability. Multivariable linear regression analysis showed that flow-mediated dilation, isoprostanes, and cotinine were independently associated with sNOX2-dp levels. Of note, sNOX2-dp serum levels were significantly higher in children with allergic rhinitis exposed to smoke, as compared with unexposed children with allergic rhinitis.CONCLUSION: NOX2 is activated in children with persistent allergic rhinitis and passive smoke exposure exacerbates this effect. We further demonstrate an association between higher sNOX2-dp and oxidative stress and endothelial dysfunction.

U2 - 10.1016/j.jpeds.2018.06.053

DO - 10.1016/j.jpeds.2018.06.053

M3 - Article

C2 - 30172425

VL - 202

SP - 252

EP - 257

JO - Journal of Pediatrics

JF - Journal of Pediatrics

SN - 0022-3476

ER -