paH gradients in isoelectric focusing experiments at subzero temperatures

Michele Perrella, Laura Cremonesi, Ilaria Vannini Parenti, Louise Benazzi, Luigi Rossi-Bernardi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

An improved method for the subzero separation of proteins by isoelectric focusing in gels of acrylamide/ethylacrylate copolymers in dimethylsulfoxide-water mixtures is described, together with the measurements of the rate of formation and stability of the paH gradient in gels under such conditions.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalAnalytical Biochemistry
Volume105
Issue number1
DOIs
Publication statusPublished - 1980

Fingerprint

Isoelectric Focusing
Gels
Temperature
Acrylamide
Dimethyl Sulfoxide
Copolymers
Experiments
Water
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

paH gradients in isoelectric focusing experiments at subzero temperatures. / Perrella, Michele; Cremonesi, Laura; Parenti, Ilaria Vannini; Benazzi, Louise; Rossi-Bernardi, Luigi.

In: Analytical Biochemistry, Vol. 105, No. 1, 1980, p. 126-132.

Research output: Contribution to journalArticle

Perrella, Michele ; Cremonesi, Laura ; Parenti, Ilaria Vannini ; Benazzi, Louise ; Rossi-Bernardi, Luigi. / paH gradients in isoelectric focusing experiments at subzero temperatures. In: Analytical Biochemistry. 1980 ; Vol. 105, No. 1. pp. 126-132.
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