paH gradients in isoelectric focusing experiments at subzero temperatures

Michele Perrella; Laura Cremonesi; Ilaria Vannini Parenti; Louise Benazzi; Luigi Rossi-Bernardi

doi:10.1016/0003-2697(80)90433-9

pa_H gradients in isoelectric focusing experiments at subzero temperatures

Michele Perrella, Laura Cremonesi, Ilaria Vannini Parenti, Louise Benazzi, Luigi Rossi-Bernardi

IRCCS Multimedica

Research output: Contribution to journal › Article

Abstract

An improved method for the subzero separation of proteins by isoelectric focusing in gels of acrylamide/ethylacrylate copolymers in dimethylsulfoxide-water mixtures is described, together with the measurements of the rate of formation and stability of the pa_H gradient in gels under such conditions.

Original language	English
Pages (from-to)	126-132
Number of pages	7
Journal	Analytical Biochemistry
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90433-9
Publication status	Published - 1980

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0003-2697(80)90433-9

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Perrella, M., Cremonesi, L., Parenti, I. V., Benazzi, L., & Rossi-Bernardi, L. (1980). pa_H gradients in isoelectric focusing experiments at subzero temperatures. Analytical Biochemistry, 105(1), 126-132. https://doi.org/10.1016/0003-2697(80)90433-9

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abstract = "An improved method for the subzero separation of proteins by isoelectric focusing in gels of acrylamide/ethylacrylate copolymers in dimethylsulfoxide-water mixtures is described, together with the measurements of the rate of formation and stability of the paH gradient in gels under such conditions.",

author = "Michele Perrella and Laura Cremonesi and Parenti, {Ilaria Vannini} and Louise Benazzi and Luigi Rossi-Bernardi",

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