paH gradients in isoelectric focusing experiments at subzero temperatures

Michele Perrella, Laura Cremonesi, Ilaria Vannini Parenti, Louise Benazzi, Luigi Rossi-Bernardi

Research output: Contribution to journalArticlepeer-review


An improved method for the subzero separation of proteins by isoelectric focusing in gels of acrylamide/ethylacrylate copolymers in dimethylsulfoxide-water mixtures is described, together with the measurements of the rate of formation and stability of the paH gradient in gels under such conditions.

Original languageEnglish
Pages (from-to)126-132
Number of pages7
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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