Pathological remodelling of colonic wall following dopaminergic nigrostriatal neurodegeneration

Carolina Pellegrini, Chiara Ippolito, Cristina Segnani, Amelio Dolfi, Mariella Errede, Daniela Virgintino, Matteo Fornai, Luca Antonioli, Francesca Garelli, Anna Nericcio, Rocchina Colucci, Silvia Cerri, Fabio Blandini, Corrado Blandizzi, Nunzia Bernardini

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aim: Patients with Parkinson's disease (PD) are often characterized by functional gastrointestinal disorders. Such disturbances can occur at all stages of PD and precede the typical motor symptoms of the disease by many years. However, the morphological alterations associated with intestinal disturbances in PD are undetermined. This study examined the remodelling of colonic wall in 6-hydroxydopamine (6-OHDA)-induced PD rats. Methods: 8 weeks after 6-OHDA injection animals were sacrificed. Inflammatory infiltrates, collagen deposition and remodelling of intestinal epithelial barrier and tunica muscularis in the colonic wall were assessed by histochemistry, immunohistochemistry, immunofluorescence and western blot analysis. Results: 6-OHDA rats displayed significant alterations of colonic tissues as compared with controls. Signs of mild inflammation (eosinophil infiltration) and a transmural deposition of collagen fibres were observed. Superficial colonic layers were characterized by severe morphological alterations. In particular, lining epithelial cells displayed a reduced claudin-1 and transmembrane 16A/Anoctamin 1 (TMEM16A/ANO1) expression; goblet cells increased their mucin expression; colonic crypts were characterized by an increase in proliferating epithelial cells; the density of S100-positive glial cells and vimentin-positive fibroblast-like cells was increased as well. Several changes were found in the tunica muscularis: downregulation of α-smooth muscle actin/desmin expression and increased proliferation of smooth muscle cells; increased vimentin expression and proliferative phenotype in myenteric ganglia; reduction of interstitial cells of Cajal (ICCs) density. Conclusions: A pathological remodelling occurs in the colon of 6-OHDA rats. The main changes include: enhanced fibrotic deposition; alterations of the epithelial barrier; activation of mucosal defense; reduction of ICCs. These results indicate that central nigrostriatal denervation is associated with histological changes in the large bowel at mucosal, submucosal and muscular level. These alterations might represent morphological correlates of digestive symptoms in PD.

Original languageEnglish
Article number104821
JournalNeurobiology of Disease
Volume139
DOIs
Publication statusPublished - Jun 2020

Keywords

  • 6-hydroxydopamine
  • Collagen deposition
  • Colon
  • Histochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Intestinal dysfunctions
  • Morphological remodelling
  • Parkinson's disease
  • Rat model

ASJC Scopus subject areas

  • Neurology

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