Patient-derived ovarian tumor xenografts recapitulate human clinicopathology and genetic alterations

Francesca Ricci, Francesca Bizzaro, Marta Cesca, Federica Guffanti, Monica Ganzinelli, Alessra Decio, Carmen Ghilardi, Patrizia Perego, Robert Fruscio, Alessro Buda, Rodolfo Milani, Paola Ostano, Giovanna Chiorino, Maria Rosa Bani, Giovanna Damia, Raffaella Giavazzi

Research output: Contribution to journalArticle

Abstract

Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy. On the basis of its histopathology and molecular-genomic changes, ovarian cancer has been divided into subtypes, each with distinct biology and outcome. The aim of this study was to develop a panel of patient-derived EOC xenografts that recapitulate the molecular and biologic heterogeneity of human ovarian cancer. Thirty-four EOC xenografts were successfully established, either subcutaneously or intraperitoneally, in nude mice. The xenografts were histologically similar to the corresponding patient tumor and comprised all the major ovarian cancer subtypes. After orthotopic transplantation in the bursa of the mouse ovary, they disseminate into the organs of the peritoneal cavity and produce ascites, typical of ovarian cancer. Gene expression analysis and mutation status indicated a high degree of similarity with the original patient and discriminate different subsets of xenografts. They were very responsive, responsive, and resistant to cisplatin, resembling the clinical situation in ovarian cancer. This panel of patient-derived EOC xenografts that recapitulate the recently type I and type II classi fication serves to study the biology of ovarian cancer, identify tumor-speci fi c molecular markers, and develop novel treatment modalities..

Original languageEnglish
Pages (from-to)6980-6990
Number of pages11
JournalCancer Research
Volume74
Issue number23
DOIs
Publication statusPublished - Dec 1 2014

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Ricci, F., Bizzaro, F., Cesca, M., Guffanti, F., Ganzinelli, M., Decio, A., Ghilardi, C., Perego, P., Fruscio, R., Buda, A., Milani, R., Ostano, P., Chiorino, G., Bani, M. R., Damia, G., & Giavazzi, R. (2014). Patient-derived ovarian tumor xenografts recapitulate human clinicopathology and genetic alterations. Cancer Research, 74(23), 6980-6990. https://doi.org/10.1158/0008-5472.CAN-14-0274