Patients affected by metabolic syndrome show decreased levels of circulating platelet derived growth factor (PDGF)-BB

Veronica Tisato, Barbara Toffoli, Lorenzo Monasta, Stella Bernardi, Riccardo Candido, Giorgio Zauli, Paola Secchiero

Research output: Contribution to journalArticle

Abstract

Background & aims: The development and/or progression of metabolic syndrome (MetS) in overweight and obese individuals have been associated to low-grade inflammation, but few studies have simultaneously analyzed the circulating levels of several cytokines. Methods: In this pilot study, a group of 27 cytokines and growth factors was analyzed in the serum of obese patients (n= 40) diagnosed for MetS in comparison with sex- and age-matched control subjects without MetS (n= 53) by using a multiplex immunoassay. Release of cytokines was measured in culture supernatants of human primary endothelial cells, THP-1 macrophagic cells and HuH-7 hepatoma cells upon exposure to a high fat mixture. Results: While the majority of cytokines did not show significant differences between the investigated groups, the circulating levels of CXCL10/IP-10 and IL-6 were higher in the MetS group versus overweight control group. In contrast, PDGF-BB serum levels were significantly decreased in MetS patients. The in vitro addition of a high fat mixture increased the release of IL-6 and/or CXCL10/IP-10 in the culture supernatant of human primary endothelial cells and THP-1 macrophagic cells, while the same mixture significantly decreased the release of PDGF-BB by human THP-1 macrophagic and HuH-7 hepatoma cells. Conclusions: The current demonstration that MetS is associated with decrease of the pro-fibrotic PDGF cytokine is a completely novel finding, which adds complexity to the interplay between inflammation and fibrosis in patients affected by MetS.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalClinical Nutrition
Volume32
Issue number2
DOIs
Publication statusPublished - Apr 2013

Keywords

  • Cytokines
  • Metabolic syndrome
  • Metabolic Syndrome
  • MetS
  • PDGF-BB

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Nutrition and Dietetics

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