Pattern of activation of human antigen presenting cells by genotype GII.4 norovirus virus-like particles

Eleonora Ponterio, Annacarmen Petrizzo, Ilaria Di Bartolo, Franco Maria Buonaguro, Luigi Buonaguro, Franco Maria Ruggeri

Research output: Contribution to journalArticle

Abstract

Background: Virus-like particles (VLPs) from an Italian GII.4 norovirus strain were used to investigate activation and maturation of circulating antigen presenting cells (APCs) of human origin.Methods: Peripheral blood mononuclear cells (PBMCs) isolated from five healthy subjects were pulsed ex vivo with VLPs, and stained with a set of monoclonal antibodies (MAbs) for phenotypic analysis by flow cytometry. Cytokine release in cell supernatants was investigated by ELISA.Results: Norovirus VLPs induced activation and maturation of circulating APCs derived from the five donors, as well as production of IL-6, IFN-γ and TNF-α cytokines.Conclusions: The present results suggest that VLPs can activate antigen presenting cells for an efficient induction of the adaptive immune response.

Original languageEnglish
Article number127
JournalJournal of Translational Medicine
Volume11
Issue number1
DOIs
Publication statusPublished - May 24 2013

Keywords

  • Immunology
  • Norovirus
  • PBMCs
  • VLPs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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