Abstract
Thirty cases of hepatocellular carcinoma (HCC) were investigated immunocytochemically for expression of β1 integrin molecule and of collagen IV. Immunoreactivity was related to the tumour proliferation index, as detected by PCNA immunostaining, and to tumour size and grade. Membrane β1 integrin immunoreactivity was detected in the neoplastic cells of all cases, though two different staining patterns were clearly recognized. In 14 cases, β1 integrin immunoreactivity was confined to the cell-stroma interface, showing the same polarized pattern as the non-neoplastic cell counterpart. This staining pattern was associated significantly (P <0.0001) with low PCNA labelling (i.e. less than 20 per cent of neoplastic cells showing nuclear immunostaining. Conversely, 16 cases showed non-polarized pericellular β1 integrin immunostaining This staining pattern was significantly associated (P <0.0001) with high PCNA labelling (more than 20 per cent of immunoreactive cells) and with tumour size greater than 4 cm in diameter (P <0.0001). β1 Integrin, collagen IV, and PCNA immunoreactivities, however, did not correlate with the histological grade. The data emphasize that neoplastic progression of HCCs may be correlated with an aberrant expression of adhesion molecules and with a disruption of the collagen IV complement of basal membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 5-11 |
| Number of pages | 7 |
| Journal | Journal of Pathology |
| Volume | 171 |
| Issue number | 1 |
| Publication status | Published - 1993 |
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Keywords
- β1 Integrin
- Collagen IV
- Hepatocellular carcinoma
- Proliferating cell nuclear antigen (PCNA)
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Patterns of integrin common chain β1 and collagen IV immunoreactivity in heptocellular carcinoma. Correlations with tumour growth rate, grade and size. / Patriarca, C.; Roncalli, M.; Gambacorta, M.; Cominotti, M.; Coggi, G.; Viale, G.
In: Journal of Pathology, Vol. 171, No. 1, 1993, p. 5-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Patterns of integrin common chain β1 and collagen IV immunoreactivity in heptocellular carcinoma. Correlations with tumour growth rate, grade and size
AU - Patriarca, C.
AU - Roncalli, M.
AU - Gambacorta, M.
AU - Cominotti, M.
AU - Coggi, G.
AU - Viale, G.
PY - 1993
Y1 - 1993
N2 - Thirty cases of hepatocellular carcinoma (HCC) were investigated immunocytochemically for expression of β1 integrin molecule and of collagen IV. Immunoreactivity was related to the tumour proliferation index, as detected by PCNA immunostaining, and to tumour size and grade. Membrane β1 integrin immunoreactivity was detected in the neoplastic cells of all cases, though two different staining patterns were clearly recognized. In 14 cases, β1 integrin immunoreactivity was confined to the cell-stroma interface, showing the same polarized pattern as the non-neoplastic cell counterpart. This staining pattern was associated significantly (P <0.0001) with low PCNA labelling (i.e. less than 20 per cent of neoplastic cells showing nuclear immunostaining. Conversely, 16 cases showed non-polarized pericellular β1 integrin immunostaining This staining pattern was significantly associated (P <0.0001) with high PCNA labelling (more than 20 per cent of immunoreactive cells) and with tumour size greater than 4 cm in diameter (P <0.0001). β1 Integrin, collagen IV, and PCNA immunoreactivities, however, did not correlate with the histological grade. The data emphasize that neoplastic progression of HCCs may be correlated with an aberrant expression of adhesion molecules and with a disruption of the collagen IV complement of basal membranes.
AB - Thirty cases of hepatocellular carcinoma (HCC) were investigated immunocytochemically for expression of β1 integrin molecule and of collagen IV. Immunoreactivity was related to the tumour proliferation index, as detected by PCNA immunostaining, and to tumour size and grade. Membrane β1 integrin immunoreactivity was detected in the neoplastic cells of all cases, though two different staining patterns were clearly recognized. In 14 cases, β1 integrin immunoreactivity was confined to the cell-stroma interface, showing the same polarized pattern as the non-neoplastic cell counterpart. This staining pattern was associated significantly (P <0.0001) with low PCNA labelling (i.e. less than 20 per cent of neoplastic cells showing nuclear immunostaining. Conversely, 16 cases showed non-polarized pericellular β1 integrin immunostaining This staining pattern was significantly associated (P <0.0001) with high PCNA labelling (more than 20 per cent of immunoreactive cells) and with tumour size greater than 4 cm in diameter (P <0.0001). β1 Integrin, collagen IV, and PCNA immunoreactivities, however, did not correlate with the histological grade. The data emphasize that neoplastic progression of HCCs may be correlated with an aberrant expression of adhesion molecules and with a disruption of the collagen IV complement of basal membranes.
KW - β1 Integrin
KW - Collagen IV
KW - Hepatocellular carcinoma
KW - Proliferating cell nuclear antigen (PCNA)
UR - http://www.scopus.com/inward/record.url?scp=0027489888&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027489888&partnerID=8YFLogxK
M3 - Article
C2 - 7693901
AN - SCOPUS:0027489888
VL - 171
SP - 5
EP - 11
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 1
ER -